TABLE 1.

Description of L. monocytogenes strains used for cell culture and animal infection experiments

Strain (previous name)Isogenic mutant pair no.aIsogenic mutant set no.bGenotype or descriptionReference
10403S11Wild-type laboratory control strain encoding a full-length InlABishop et al. (3)
EGD-eLaboratory control strain encoding a full-length InlA that carries a homologous 3′ inlA allelic type to ribotype DUP-1062A isolates with the exception of the mutation leading to inlA PMSC type 3Glaser et al. (15)
FSL W3-084 (HEL-399)11Isogenic mutant where premature inlA stop codon mutation type 3 was introduced in a 10403S backgroundNightingale et al. (33)
FSL K4-006ΔinlA null mutant in a 10403S backgroundKim et al. (22)
FSL K4-007ΔinlB null mutant in a 10403S backgroundKim et al. (22)
FSL K4-009ΔinlAB null mutant in a 10403S backgroundKim et al. (22)
FSL F2-51521Natural ribotype DUP-1062A isolate with premature inlA stop codon mutation type 3Nightingale et al. (33)
FSL N4-73021Isogenic mutant where premature inlA stop codon mutation type 3 was reverted in a FSL F2-515 backgroundThis paper
FSL F2-56332Natural ribotype DUP-1052A isolate with premature inlA stop codon mutation type 1Nightingale et al. (33)
FSL N4-73432Isogenic mutant where premature inlA stop codon mutation type 1 was reverted in a FSL F2-563 backgroundThis paper
FSL F2-24542Wildtype DUP-1052A isolate with inlA homologous to FSL F2-563 with the exception of premature inlA stop codon mutation type 1 and thus encoding a full-length InlANightingale et al. (33)
FSL N4-73342Isogenic mutant where premature inlA stop codon mutation type 1 was introduced in a FSL F2-245 backgroundThis paper
  • a Paired isogenic mutants that only differ by the presence or absence of each respective premature stop codon mutation in inlA.

  • b A set of four isogenic mutants assembled to evaluate the virulence of each respective premature stop codon in inlA. Each mutant set includes a natural isolate carrying a PMSC mutation and its revertant along with a wild-type strain encoding a full-length inlA and its paired mutant, where a given premature stop codon in inlA was introduced into its parent wild-type strain.