TABLE 2.

Competition by other carbohydrates for glucose uptake in B. animalis subsp. lactis DSMZ 10140

Competitor (assayed at 30 mM)a% Change in glucose uptakeb
None0 ± 2c
d-Ribose−2 ± 2
d-Melibiose−7 ± 2*
Methyl-α-glucoside−11 ± 3*
Maltose−13 ± 5*
2-Deoxy-d-glucose−20 ± 3*
d-Galactose−23 ± 3*
N-Acetylglucosamine−25 ± 8*
Methyl-β-glucoside−35 ± 1*
  • a Glucose uptake was determined by growing DSMZ 10140 in LG broth to mid-log phase. Glucose uptake assays were performed in 0.05 M potassium phosphate buffer containing 5 mM NaCl, 1 mM glucose, and 30 mM competitor.

  • b Values represent means ± standard deviations from duplicate assays and at least two replications. Values with an asterisk are statistically different from the control without added competitor (α = 0.05).

  • c Mean value for control without added competitor, 7.0 nmol/min/mg cell protein.