TABLE 2.

Seventy novel L. pneumophila genes affecting amoeba infection identified by means of the scatter screena

Locus tagClosest homolog (organism)E value
lpg0026Amino acid permease (Cellvibrio japonicus)5.00E−132
lpg0072Excinuclease ABC subunit B (Pseudomonas mendocina)0.0
lpg0129Methylmalonate-semialdehyde dehydrogenase (Aeromonas salmonicida)1E−164
lpg0166Putative membrane protein (Acinetobacter baumannii)2E−83
lpg0200Cytochrome d ubiquinol oxidase, subunit II (Rickettsiella grylli)1E−100
lpg0230PleD-like protein (Synechocystis sp.)8E−79
lpg0233Thiamine pyrophosphate protein binding domain protein (Methylobacterium extorquens)2E−71
lpg0237Lipolytic enzyme (Nodularia spumigena)2E−45
lpg0243Probable short-chain dehydrogenase (Pseudomonas aeruginosa)6E−68
lpg0265Putative multicopper oxidase (Methylobacterium nodulans)4E−90
lpg0277Hypothetical protein BuboB_27536 (Burkholderia ubonensis)1.00E−153
lpg0493Amino acid ABC transporter, ATP binding protein (Wolbachia endosymbiont of Drosophila ananassae)5E−67
lpg0515Hypothetical protein FG10257.1 (Gibberella zeae)2E−28
lpg0549Gamma-glutamyltranspeptidase (Methylococcus capsulatus)4E−150
lpg0599Putative poly-beta-hydroxybutyrate synthase (Azoarcus sp. strain EbN1)5E−166
lpg0618Probable 3-methyladenine-DNA glycosylase I (“Candidatus Protochlamydia amoebophila”)2E−66
lpg0635Probable melitin resistance protein (Chromobacterium violaceum)0.0
lpg0641Heat shock protein HslVU, ATPase subunit HslU (Hahella chejuensis)0.0
lpg0685Hypothetical protein Nmul_A1979 (Nitrosospira multiformis)2E−171
lpg1019Heavy metal RND efflux membrane fusion protein, CzcB family (Pseudomonas stutzeri)2E−71
lpg1110None
lpg1121None
lpg1146Zn-dependent carboxypeptidase (Yersinia frederiksenii)5.00E−131
lpg1188Potassium uptake protein, Kup system (Geobacter sulfurreducens)8E−147
lpg1349Apolipoprotein N-acyltransferase (Rickettsiella grylli)9.00E−83
lpg1373RNase HII (Haemophilus ducreyi)2E−59
lpg1376Hypothetical protein MED92_07681 (Oceanospirillum sp.)2.00E−42
lpg1426Hypothetical protein RT0522 (Rickettsia typhi strain Wilmington)2.00E−17
lpg1441Putative metal-dependent phosphoesterase (marine gammaproteobacterium strain HTCC2207)6.00E−58
lpg1466Hypothetical protein Rfer_2906 (Rhodoferax ferrireducens)1.00E−21
lpg1469Peptidase S13 d-Ala-d-Ala carboxypeptidase C (Burkholderia phytofirmans)7.00E−154
lpg1507Sodium/hydrogen antiporter family protein (Coxiella burnetii)1.00E−116
lpg1596Putative fatty oxidation complex, alpha subunit (Coxiella burnetii strain Dugway)0.0
lpg1610Glutamate 5-kinase, ProB related (Prosthecochloris aestuarii)5.00E−69
lpg1615ABC-type multidrug transport system, ATPase component (Rickettsia bellii)6E−142
lpg1634Flavin adenine dinucleotide-linked oxidase domain protein (Cyanothece sp. strain PCC 8801)0.0
lpg1638Integral membrane protein (Streptomyces coelicolor)6.00E−78
lpg1653d-Xylose-proton symporter (Rickettsiella grylli)4.00E−81
lpg1665Alpha-2-macroglobulin domain protein (Burkholderia cenocepacia)0.0
lpg1702Kinectin 1 (kinesin receptor) (Legionella pneumophila Philadelphia-1)1E−61
lpg1762Two-component response regulator (Oceanospirillum sp. strain MED92)5.00E−138
lpg1805DNA mismatch repair protein MutS (Coxiella burnetii)0.0
lpg1843Peptidase S33, proline iminopeptidase 1 (marine gammaproteobacterium strain HTCC2143)2.00E−99
lpg1925None
lpg1974Hypothetical protein Pcar_0519 (Pelobacter carbinolicus)2.00E−11
lpg2011Hypothetical protein Noc_0956 (Nitrosococcus oceani)2.00E−65
lpg2031Arginyl-tRNA synthetase (Methylococcus capsulatus strain Bath)0.0
lpg2032Hypothetical protein SIAM614_20101 (Stappia aggregata)3.00E−59
lpg2132Diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (Desulfuromonas acetoxidans)3E−63
lpg2257Hypothetical protein B14911_25090 (Bacillus sp. strain NRRL B-14911)4.00E−05
lpg23163-HB dehydrogenase (Delftia acidovorans)1.00E−112
lpg2339Conserved hypothetical protein (Mycobacterium marinum)5.00E−35
lpg2401Beta-lactamase (Flavobacterium johnsoniae)3.00E−15
lpg2409None
lpg2494Kynurenine formamidase (Methylokorus infernorum)1.00E−25
lpg2522Metal-activated pyridoxal enzyme (Moritella sp. strain PE36)1.00E−99
lpg2527None
lpg2538None
lpg2544Transglycosylase, putative (Desulfovibrio desulfuricans)7.00E−23
lpg2577None
lpg2680Cyanophycin synthetase (Clostridium thermocellum ATCC 27405)2.00E−37
lpg2806None
lpg2814Leucine aminopeptidase, putative (Burkholderia thailandensis)1.00E−52
lpg2815Putative membrane protein (Rickettsiella grylli)4.00E−34
lpg2832Hypothetical protein Mmc1_1487 (Magnetococcus sp. strain MC-1)2.00E−21
lpg2835Thiopurine S-methyltransferase (Shewanella baltica)3.00E−37
lpg2870Hypothetical protein PE36_07941 (Moritella sp. strain PE36)1.00E−39
lpg2885None
lpg2917Conserved hypothetical protein (Mycobacterium abscessus)2.00E−91
lpg2996Predicted methyltransferase (Hahella chejuensis)1.00E−72
  • a Proteins with no significant homology showed expected values of ≥0.01.