TABLE 4.

Classification of OTUs clustering with free-living protozoa obtained from treated water and from biofilms in the distribution systems of water supply A and water supply Ba

Organism(s) with highest similarity (GenBank accession no.)bSimilarity (%)No. of OTUsNo. of clones
TW-ABF-AcTW-BBF-Bc
Amoebozoa103428
    Acanthamoeba polyphaga (AF260725)89.311
    Echinamoeba thermarum (AJ489264)85.711
    Eimeriidae environmental sample clone (EF024503)/Acanthamoeba sp. (AY173000)96.8/85.912
    Uncultured endolithic amoeba (AB257667)/Hartmannellidae sp. strain LO57N/1 (AY145442)79.2/77.917
    Neoparamoeba aestuarina (AY121851)89.311
    Pterocystis tropica (AY749612)93.411
    Raineriophrys erinaceoides (AY749633)94.7, 93.322
    Uncultured eukaryote clone (AY749523)/Raineriophrys sp. (AY749606)89.0/88.411
    Uncultured Sarcosomataceae clone (EF023269)/Amastigomonas mutalitis (AY050182)78.3/77.111
Cercozoa391460244
    Athalamea environmental sample clone (EF024169)/soil flagellate AND25 (AY965868)91.1/88.512
    Athalamea environmental sample clone (EF024169)/Hedriocystis reticulata (AY305010)90.3/85.011
    Athalamea environmental sample clone (EF024169)/Exuviaella pusilla (DQ388459)81.4/81.011
    Cercomonas longicauda (AY496047, AF411270, AY496047)96.3, 94.6, 91.9341
    Cercomonas metabolicus (DQ211597)97.5, 95.6, 94.836
    Cercomonas sp. (AF534712)95.2, 95.0, 80.6335
    Cercomonadida environmental sample clone (EF024293 and EF024163)/soil flagellate AND24 (AY965867)98.1, 93.4/94.1, 92.6211
    Cercomonadidae environmental sample clone (EF024692)/Cercomonas sp. (AF411266)95.1/95.011
    Cercomonadida environmental sample clone (EF024163)/Masisteria marina strain DFS1 (AF174371)87.4/84.611
    Dimorpha-like sp. strain ATCC 50522 (AF411283)93.4110
    Dodomorpha sp. strain HFCC57 (DQ211596)99.4124
    Ebria triparrtita (DQ303922)88.213
    Pseudodiffllugia cf. gracilis (AJ418794)88.711
    Trachelocorythion pulchellum (AJ418789)76.5113
    Uncultured Banisveld eukaryote clone (EU091827)/soil flagellate AND21 (AY965866)99.8/99.111
    Uncultured cercozoan sample clone (EF023523)/Cercomonadida environmental sample clone (EF024163)92.6/91.912
    Uncultured cercozoan clone (AY620301)/Cercomonas sp. (AF411266)98.0/91.411
    Uncultured cercozoan clone (AY620269)/Massisteria marina (AF174373)89.1/87.411
    Uncultured cercozoan clone (AY821946)/Pseudodiffllugia cf. gracilis (AJ418794)90.0/87.512
    Uncultured cercozoan partial 18S rRNA gene (AM114807)/soil flagellate AND24 (AY965867)99.0/93.013
    Uncultured cercozoan clone (AY620268)/Cercomonas sp. (AF411266)97.9/89.111
    Uncultured eukaryote clone (AY082981)/Ebria tripartita (DQ303922)96.1/88.011
    Uncultured eukaryote clone (EF024996)/Protaspis grandis (DQ303924)96.1/92.611
    Uncultured eukaryote clone (AY082993)/uncultured freshwater cercozoan (DQ243993)95.1/94.011
    Uncultured eukaryote clone (EF023764)/Protaspis grandis (DQ303924)99.4/94.011
    Uncultured freshwater cercozoan clone (DQ244000)/Cercomonadidae environmental sample clone (EF024294)83.3/81.411
    Uncultured freshwater cercozoan clone (DQ243992 and (DQ243993)/Cercomonas sp. (AF411271 and AF411266)92.6, 89.0/91.4, 88.5221
    Uncultured freshwater cercozoan clone (DQ243993)/Rigidomastix-like sp. (AF411279)93.5/92.911
    Uncultured rhizosphere cercozoan (AJ506007)/soil flagellate AND21 (AY905866)78.9/74.511
    Unidentified eukaryote (AJ130856)/Lecythium sp. (AJ514867)97.0, 95.9/95.9, 94.423
Choanozoa263151413
    Amoebidium parasiticum strain ATCC 32708 (Y19155)90.511
    Codonosigidae evironmental sample clone (EF024012)/Monosiga ovata (AF271999)97.1/93.511
    Corallochytrium limacisporum (L42528)79.511
    Diaphanoeca grandis (DQ103820, AY753614, AF084234)92.9, 82.8, 75.7333
    Eimeriidae environmental sample clone (EF024885)/Diaphanoeca grandis (AF084234)92.3/90.111
    Eimeriidae environmental sample clone (EF024885)/Endochytrium sp. (AY635844)91.9/90.211
    Eimeriidae environmental sample clone (EF023936)/Monosiga ovata (AF084230)94.9, 94.1, 90.2/92.1, 91.4, 88.9321
    Eimeriidae environmental sample clone (EF024885)/Stephanoeca diplocostata (AF084235 and AY149899)91.5, 93.5/90.2, 92.8271
    Ichthyophonus irregularis (AF232303 and AF232303)92.4, 79.4221
    Nuclearia moebiusi (AF484686)91.811
    Rhinosporidium seeberi (AF118851)90.111
    Uncultured eukaryote (AB275066)/Diaphanoeca grandis (AF684234)90.7/89.611
    Uncultured eukaryotic picoplankton clone (AY642728)/Monosiga ovata (AF084230)94.8, 98.5, 90.7/94.4, 90.4351
    Uncultured eukaryotic picoplankton clone (AY642707)/Stephanoeca diplocostata (AY149899)92.2/92.011
    Uncultured marine eukaryote clone (EF526879)/Corallochytrium limacisporum (L42528)90.4/89.912
    Uncultured marine eukaryote clone (EF526803 and DQ103820)/Diaphanoeca grandis (AF084234 and L10824)94.0, 88.7/92.4, 88.4214
    Uncultured marine eukaryote clone (EF526803)/Stephanoeca diplocostata (AY149899)93.4/92.013
Ciliophora29741721
    Dexitrichides pangi (AY212805)91.212
    Heliophrya erhardi (AY007445)86.3, 85.625
    Hemiophrys macrostoma (AY102173)82.611
    Hemiophrys procera (AY102175)98.1, 96.822
    Holosticha diademata (DQ059583)98.7, 96.722
    Oxytrichidae environmental sample clone (EF024903)/Gonostomum namibiense (AY498655)97.5/96.911
    Parabirojimia similis (DQ503584)97.311
    Tokophrya lemnarum clone (AY332720)87.614
    Unidentified eukaryote (AJ130855)/Carchesium polypinum (AF401522)90.4/90.211
    Uncultured eukaryote clone (EF024996)/Dexitrichides pangi (AY212805)98.9, 87.111
    Unidentified eukaryote (AJ130851)/Ophrydium versatile (AF401526)85.9, 85.311
    Unidentified eukaryote (AJ130851)/Vorticella campanula (AF335518 and DQ662849)94.8, 92.4/94.6, 92.124
    Uncultured hypotrichid ciliate clone (AY821937)/Aspidisca steini (AF305625)88.9/87.712
    Uncultured marine eukaryote clone (DQ103847)/uncultured ciliate (AM114813)77.0/76.213
    Uncultured marine eukaryote clone (EF526916)/Plagiopyliella pacifica (AY541685)94.5/94.512
    Uroleptus gallina (AF164130)87.611
    Vorticella campanula (DQ662849 and AF335518)99.2, 98.4, 93.6, 95.2, 93.4, 91.6617
    Vorticella fusca (DQ190468)99.311
    Vorticella sp. strain JCC-2006-4 (DQ868349)96.6151
    Zoothamnium niveum (DQ868350)94.811
Euglenozoa1317120
    Bodo saltans (DQ207571); (AY490229)95.6, 77.222
    Neobodo designis (AY753616, AY753616, DQ207583)97.4, 94.3, 93.0, 91.1, 88.3, 87.76101
    Petalomonas cantuscygni CCAP 1259/1 (AF386635)85.811
    Rhynchomonas nasuta (DQ207595 and AY425023)98.1; 85.424
    Uncultured eukaryote clone (AY753980)/Bodo saltans (AY490232)94.5/93.611
    Uncultured eukaryote clone (EF100316)/Petalomonas cantuscygni (U84731)80.5/78.511
Myzozoa501111
    Pseudoperkinsus tapetis (AB300505)86.811
    Uncultured alveolate clone (AF372776)/Corallochytrium limacisporum (L42528)87.8/78.911
    Uncultured eukaryote clone (EF100258)/uncultured alveolate clone (AF372776)97.3/91.019
    Uncultured eukaryote clone (EF100258)/Colpodella pontica (AY078092)98.1/77.311
    Uncultured marine eukaryote clone (DQ103862)/Peridinium wierzejskii (AY443018)97.1/96.711
Stramenopiles50412
    Aphanomyces invadans (DQ403202 and AF396684)98.7, 98.423
    Hyphochytrium catenoides (AF163294)97.312
    Paraphysomonas foraminifera (AB022864)98.511
    Rhizidiomyces apophysatus (AF163295)98.311
Total12744897149
  • a OTUs were compared with sequences in the ARB database (10, 38).

  • b Most closely related sequence/most closely related genus or species. More than one accession number indicates that more than one OTU had the highest similarity to the same genus or species, but to different sequence types.

  • c Data are totals for the three analyzed biofilm samples.