TABLE 1.

Bacterial strains, plasmids, and primers used in this study

Strain, plasmid, or primerRelevant characteristics or sequenceReference or source
E. coli strains
    DH5αF′ endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 (lacZYA-argF)U169 deoR [80dlac(lacZ)M15recA1] 44
    JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB) mcrA [F′ traD36 proAB lacIqlacZΔM15 63
C. violaceum CV026Double mini-Tn5 mutant from C. violaceum ATCC 31532, AHL biosensor 36
P. putida F117AHL-negative derivative of P. putida IsoF; PpuI 50
P. aeruginosa strains
    PAO1Wild type 21
    PAO1lasI::GmPAO1 with Gm cartridge inserted into unique EcoRI site of lasI 3
    PAO1rhlI::TcPAO1 with Tc cartridge inserted into unique EcoRI site of rhlI 3
    PAO1rsaL rsaL::ISlacZ-hah 42
    PUPa3Wild type, rice rhizosphere isolate 28
    LASI lasI::Km of P. aeruginosa PUPa3; KmrThis study
    LASR lasR::Km of P. aeruginosa PUPa3; KmrThis study
    RHLI rhlI::Gm of P. aeruginosa PUPa3; GmrThis study
    DMI lasI::Km rhlI::Gm of P. aeruginosa PUPa3; Kmr GmrThis study
    DMR lasR::Km rhlR::Tet of P. aeruginosa PUPa3; Kmr TetrThis study
    RSAL rsaL::Tc of P. aeruginosa PUPa3; TetrThis study
Plasmids
    pKRC12pBBR1MCS-5 carrying PlasB-gfp (ASV) Plac-lasR; Gmr 43
    pSB1075 lasR-PlasI luxCDABE; Ampr 61
    pRK2013Tra+ Mob+ ColE1 replicon; Kmr 15
    pMOSBlueCloning vector; AmprAmersham-Pharmacia
    pBluescript KSCloning vector; AmprStratagene
    pLAFR3Broad-host-range cloning vector, IncP1; Tetr 49
    pIB101pLAFR3 containing P. aeruginosa PUPa3 DNAThis study
    pIB103pLAFR3 containing P. aeruginosa PUPa3 DNAThis study
    pMULTIAHLPROMBroad-host-range plasmid containing eight luxI-type promoters fused to a promoterless lacZ gene; Tetr 52
    pKNOCK-KmConjugative suicide vector; Kmr 1
    pKNOCK-GmConjugative suicide vector; Gmr 1
    pKNOCK-TetConjugative suicide vector; Tetr 1
    pKNOCK-lasIInternal PCR fragment of P. aeruginosa PUPa3 lasI cloned in pKNOCK-KmThis study
    pKNOCK-rhlIInternal PCR fragment of P. aeruginosa PUPa3 rhlI cloned in pKNOCK-GmThis study
    pKNOCK-rhlRInternal PCR fragment of P. aeruginosa PUPa3 rhlR cloned in pKNOCK-TetThis study
    pKNOCK-rsaLInternal PCR fragment of P. aeruginosa PUPa3 rsaL cloned in pKNOCK-TetThis study
Primers
    lasI-forGAAATCGATGGTTATGACGCThis study
    lasI-revCGGCACGGATCATCATCTTCThis study
    rhlI-forTCAGGTCTTCATCGAGAAGCThis study
    rhlI-revCGTTGCGAACGAAATAGCGThis study
    rhlR-forTGGATCCGGCGATCCTCAACThis study
    rhlR-revGCTCTAGAGCTTCTGGGTCAGCAACTThis study
    rsaL-forTTGGATCCACCCGCACCGCCCGACThis study
    rsaL-revGCTCTAGATATATAGGGAAGGGCAGGThis study