TABLE 2.

Substrate specificity of LinR

Substrateβ-Galactosidase activity (U/min/mg of protein)a
2,5-DCHQ22 ± 7.5
CHQ31 ± 14
HQ0.8 ± 0.1
2,6-DCHQ3.9 ± 1.0
TetrachloroHQ1.3 ± 0.1
Catechol1.1 ± 0.2
3-Cl catechol1.1 ± 0.1
4-Cl catechol1.1 ± 0.2
Phenol1.2 ± 0.2
m-Chlorophenol1.2 ± 0.1
o-Chlorophenol1.2 ± 0.3
Protocatechuate1.1 ± 0.1
Gentisate1.2 ± 0.1
Salicylate1.2 ± 0.2
5-Cl salicylate1.2 ± 0.2
  • a E. coli MV1190(pMEU1R) was used for the assay.