TABLE 1

Strains and plasmids used in this work

Strain or plasmidDescriptionReference or source
Strains
    SL1344hisG rpsL xyl Strr46
    CMA820SL1344 ΔbolA::CatrThis study
    CMA821CMA820 carrying the pRMA04 plasmidThis study
Plasmids
    pKD3Plasmid encoding FRT-flanked Cat cassette; pANTSγ, Ampr Catr38
    pKD46Temperature-sensitive λ-Red recombinase expression plasmid; oriR101 Ampr38
    pWKS29Low-copy-number plasmid; pSC101, Ampr47
    pRMA04pWSK29 carrying bolA, AmprThis study