TABLE 1.

Substrate tests with strain FW-1a in pure culture at 85°C

CategorySubstrate (concna)
Electron donor
    UtilizedH2 (as H2-CO2; 80:20, vol/vol; 110 kPa)
    Not utilizedLactate (10), pyruvate (10), acetate (5), malate (10), succinate (10), peptone (0.1%), formate (10), fumarate (10), yeast extract (0.1%), glycerol (20), methanol (20)b, ethanol (20)b, citrate(10), alanine (11.2), histidine (6.4), proline (8.7), glycine (13.3), isoleucine (7.6), aspartic acid (7.5), glutamic acid (6.8), arginine (5.7), l-cysteine (8.3), serine (9.5), asparagine (5.7), palmitate (1), stearate (1), valerate (1-10), butyrate (5), propionate (5), catechol (1), phenol (0.25-0.50)b, toluene (1)b, benzoate (0.5-1.0)b, benzoic acid (0.5-1.0 mM)b, benzene (0.5-1.0 mM)b
Electron acceptor
    UtilizedPoorly crystalline Fe(III) (100)
    Not utilizedSO42− (10), S2O32− (10), SO32− (10), S0 (2%, wt/vol), NO3 (10), NO2 (1.0-1.5), O2 (0.6-1.0% evaluated), MnO2 [poorly crystalline Mn(IV) (20)], 2,6-anthraquinone disulfonate (5), malate (10), fumarate (10-50), ferric pyrophosphate (10), ferric citrate (10-50), goethite (50), hematite (50), Co(III)-EDTA (200 μM), selenate (10), selenite (10), dimethyl sulfoxide (1-2)
  • a Concentrations are in millimolar unless otherwise indicated.

  • b Tested at 70 to 75°C.