TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference
S. paucimobilis
    UT26Nalr4
    UT102Nalr Kmr; Tn5-induced mutant of UT2610
E. coli
    JM110dam dcm supE hsdR17 thi leu rpsL1 lacY galK galT ara tonA thr tsx Δ(lac-proAB)/F′ [traD36 proAB+ lacIq lacZΔM15]6
    MV1190Δlac-proAB thi supE Δsrl-recA306::Tn10 F′ traD36 proAB lacI ZΔM156
Plasmids
    pMC1403pMB1 replicon; Apr18
    pHSG399pMB9 replicon; Cmr19
    pUC18pMB9 replicon; Apr19
    pUC19pMB9 replicon; Apr19
    pLR1pUC18/HincII + blunted PstI-SmaI fragment containing linR; linR is the same orientation as the lac promoterThis study
    pMEU1pMC1403 carrying 238-bp EcoRI-BamHI fragment containing the upstream region of linEThis study
    pMEU2pMC1403 carrying 163-bp EcoRI-BamHI fragment containing the upstream region of linEThis study
    pMEU3pMC1403 carrying 138-bp EcoRI-BamHI fragment containing the upstream region of linEThis study
    pMEU1RpMEU1 carrying PvuII-PvuII fragment of pLR1 at its BalI siteThis study
    pMEU2RpMEU2 carrying PvuII-PvuII fragment of pLR1 at its BalI siteThis study
    pMEU3RpMEU3 carrying PvuII-PvuII fragment of pLR1 at its BalI siteThis study