TABLE 2

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Bacterial strains
    C. acetobutylicum
        824ATCC 824, wild-type strainATCC
        ΔccpAccpA::intron14
        824-PATCC 824 carrying the pIMP1-Pthl plasmidThis study
        824-ccpAATCC 824 carrying the pIMP1-Pthl-ccpA plasmidThis study
        824-ccpA-mATCC 824 carrying the pIMP1-Pthl-ccpAmut plasmidThis study
        824 m-P-ccpAΔccpA carrying the pIMP1-PccpA-ccpA plasmidThis study
        824 m-Pm-ccpAΔccpA carrying the pIMP1-PccpAmut-ccpA plasmidThis study
        creP/824ATCC 824 carrying the pIMP1-PccpA-lacZ plasmidThis study
        creP-m/824ATCC 824 carrying the pIMP1-PccpAmut-lacZ plasmidThis study
        creORF/824ATCC 824 carrying the pIMP1-PccpA-ccpA-lacZ plasmidThis study
        creORF-m/824ATCC 824 carrying the pIMP1-PccpA-ccpAmut-lacZ plasmidThis study
        creORF/ΔccpAΔccpA carrying the pIMP1-PccpA-ccpA-lacZ plasmidThis study
        creORF-m/ΔccpAΔccpA carrying the pIMP1-PccpA-ccpAmut-lacZ plasmidThis study
    E. coli
        Top10General cloning host strainInvitrogen
        ER2275Strain used to methylate the expression vectorNew England Biolabs
        Rosetta(DE3)Strain used for protein overexpressionNovagen
Plasmids
    pIMP1-PthlVector with thl promoterThis study
    pIMP1-Pthl-ccpAccpA overexpression vector derived from pIMP1-PthlThis study
    pIMP1-Pthl-ccpAmutccpAmut overexpression vector derived from pIMP1-PthlThis study
    pIMP1-PccpA-ccpAccpA overexpression vector derived from pIMP1-PccpAThis study
    pIMP1-PccpAmut-ccpAccpA overexpression vector derived from pIMP1-PccpAmutThis study
    pIMP1-PccpA-lacZlacZ reporter gene driven by ccpA promoterThis study
    pIMP1-PccpAmut-lacZlacZ reporter gene driven by mutant ccpA promoterThis study
    pIMP1-PccpA-ccpA-lacZFusion of a segment of the ccpA gene and lacZ reporter driven by the ccpA promoterThis study
    pIMP1-PccpA-ccpAmut-lacZFusion of a segment of the ccpAmut gene and lacZ reporter driven by the ccpA promoterThis study
    pIMP1-PccpAmut-ccpAmut-lacZFusion of a segment of the ccpAmut gene and lacZ reporter driven by the ccpAmut promoterThis study