Primers used in this work
| Primer | Sequence (5′ → 3′) | Length (bp) | Tm (°C)a | Source |
|---|---|---|---|---|
| T7 promoter | TAATACGACTCACTATAGGG | 20 | 53.2 | Eurofins MWG (Ebersberg, Germany) |
| T7 terminator | CTAGTTATTGCTCAGCGGT | 19 | 54.5 | Eurofins MWG (Ebersberg, Germany) |
| PET5_for | CGCCGCCATATGAATAAATCTATTCTAAAAAAACTCTC | 38 | 68 | This work |
| PET5_rev | CGATTCGGCGGCCGCGTAATTACATGTGTCACGG | 34 | 77 | This work |
| PET6_for | CGTAGTCATATGGTACCGTGTTCGGACTG | 29 | 69 | This work |
| PET6_rev | CAGCGGCCGCCTAATAGTAACTACAGTTGTCTCG | 34 | 73 | This work |
| PET12_for | CGCCATATGCAGACCAACCCCTACCAGCGAGGCCC | 35 | 80 | This work |
| PET12_rev | CTTGCGGCCGCTCAGTACGGGCAGCTCTCGCGGTACTCC | 39 | 84 | This work |
↵a Tm, melting temperature.