TABLE 4

Primers used in this work

PrimerSequence (5′ → 3′)Length (bp)Tm (°C)aSource
T7 promoterTAATACGACTCACTATAGGG2053.2Eurofins MWG (Ebersberg, Germany)
T7 terminatorCTAGTTATTGCTCAGCGGT1954.5Eurofins MWG (Ebersberg, Germany)
PET5_forCGCCGCCATATGAATAAATCTATTCTAAAAAAACTCTC3868This work
PET5_revCGATTCGGCGGCCGCGTAATTACATGTGTCACGG3477This work
PET6_forCGTAGTCATATGGTACCGTGTTCGGACTG2969This work
PET6_revCAGCGGCCGCCTAATAGTAACTACAGTTGTCTCG3473This work
PET12_forCGCCATATGCAGACCAACCCCTACCAGCGAGGCCC3580This work
PET12_revCTTGCGGCCGCTCAGTACGGGCAGCTCTCGCGGTACTCC3984This work
  • a Tm, melting temperature.