TABLE 1.

Temporal responses of Pseudomonas sp. strains ADP and ADP M13-2 to s-triazines

AttractantaMean smooth-swimming response timeb ±SD (no. of independent assays)
ADPADP M13-2 [(NH4)2SO4]
Atrazine(NH4)2SO4
None− (5)− (5)− (5)
CAA89 ± 16 (31)113 ± 29 (19)83 ± 22 (16)
Atrazine14 ± 8 (24)18 ± 5 (16)29 ± 7 (15)
N-Isopropylammelide24 ± 8 (21)45 ± 12 (16)44 ± 10 (15)
Cyanuric acid18 ± 4 (15)17 ± 3 (15)17 ± 3 (15)
Ametryn13 ± 7 (16)28 ± 6 (16)27 ± 5 (15)
  • a Attractants were supplied at the following concentrations: Casamino Acids (CAA), 0.2%; atrazine, 0.075 mM; N-isopropylammelide, 10 mM; cyanuric acid, 5 mM; ametryn, 0.4 mM. The s-triazine concentrations used were approximately half-saturating, except for that of cyanuric acid.

  • b The amount of time in seconds for approximately 50% of the cells to adapt to the added attractant. The bacterial strains Pseudomonas sp. strains ADP (wild type) and ADP M13-2 (cured strain) were grown as described in Materials and Methods, with the nitrogen source indicated. −, no response detected.