TABLE 1.

TaqMan primers and probes used to quantify cell numbers in this study

Primer/ probeSequenceTarget gene(s)Reference or source
DhcF5′-GGT AAT ACG TAG GAA GCA AGC GDehalococcoides 16S rRNA genes18
DhcR5′-CCG GTT AAG CCG GGA AAT TDehalococcoides 16S rRNA genes18
DhcProbe5′-VIC-ACA TCC AAC TTG AAA GAC CAC CTA CGC TCA CT-TAMRADehalococcoides 16S rRNA genes18
BacF15′-TCC TAC GGG AGG CAG CAGBacteria 16S rRNA genes18
BacR15′-GGA CTA CCA GGG TAT CTA ATC CTG TTBacteria 16S rRNA genes18
BacR25′-GGA CTA CCA GAG TAT CTA ATT CTG TTBacteria 16S rRNA genes18
BacProbe5′-FAM-CGT ATT ACC GCG GCT GCT GGC AC-TAMRABacteria 16S rRNA genes18
tceAF5′-ATC CAG ATT ATG ACC CTG GTG AAtceA gene21
tceAR5′-GCG GCA TAT ATT AGG GCA TCT TtceA gene21
tceAProbe5′-FAM-TGG GCT ATG GCG ACC GCA GG-TAMRAtceA gene21
dceA1F5′-GGG TAC ACC CGA AGA AAA TCT GdceA1 geneThis study
dceA1R5′-AGC CGC CAA AAA ACC TGA TdceA1 geneThis study
dceA1Probe5′-FAM-AAA CCT GCC GTA CTG C-MGBadceA1 geneThis study
  • a MGB, minor groove binder.