TaqMan primers and probes used to quantify cell numbers in this study
| Primer/ probe | Sequence | Target gene(s) | Reference or source |
|---|---|---|---|
| DhcF | 5′-GGT AAT ACG TAG GAA GCA AGC G | Dehalococcoides 16S rRNA genes | 18 |
| DhcR | 5′-CCG GTT AAG CCG GGA AAT T | Dehalococcoides 16S rRNA genes | 18 |
| DhcProbe | 5′-VIC-ACA TCC AAC TTG AAA GAC CAC CTA CGC TCA CT-TAMRA | Dehalococcoides 16S rRNA genes | 18 |
| BacF1 | 5′-TCC TAC GGG AGG CAG CAG | Bacteria 16S rRNA genes | 18 |
| BacR1 | 5′-GGA CTA CCA GGG TAT CTA ATC CTG TT | Bacteria 16S rRNA genes | 18 |
| BacR2 | 5′-GGA CTA CCA GAG TAT CTA ATT CTG TT | Bacteria 16S rRNA genes | 18 |
| BacProbe | 5′-FAM-CGT ATT ACC GCG GCT GCT GGC AC-TAMRA | Bacteria 16S rRNA genes | 18 |
| tceAF | 5′-ATC CAG ATT ATG ACC CTG GTG AA | tceA gene | 21 |
| tceAR | 5′-GCG GCA TAT ATT AGG GCA TCT T | tceA gene | 21 |
| tceAProbe | 5′-FAM-TGG GCT ATG GCG ACC GCA GG-TAMRA | tceA gene | 21 |
| dceA1F | 5′-GGG TAC ACC CGA AGA AAA TCT G | dceA1 gene | This study |
| dceA1R | 5′-AGC CGC CAA AAA ACC TGA T | dceA1 gene | This study |
| dceA1Probe | 5′-FAM-AAA CCT GCC GTA CTG C-MGBa | dceA1 gene | This study |
↵a MGB, minor groove binder.