TABLE 1.

Essential genes identified in this study as determinants of yeast resistance to inhibitory ethanol concentrationsa

Gene/ORF nameDescription of protein
Transcription machinery and RNA processing
    BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB); involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
    CSL4Subunit of the exosome, which is an essential complex present in both the nucleus and cytoplasm that mediates RNA processing and degradation
    CWC25Component of a complex containing Cef1p, involved in pre-mRNA splicing
    HTS1Cytoplasmic and mitochondrial histidine tRNA synthetase
    IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms
    MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
    MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor); plays a role in 3′ end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA; contains a putative RNA-binding zinc knuckle motif
    PRP11Subunit of the SF3a splicing factor complex, required for spliceosome assembly
    RRP3Involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA
    SPP381mRNA splicing factor, component of U4/U6/U5 tri-snRNP
    TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes
    FHL1Putative transcriptional regulator with similarity to DNA-binding domain of Drosophila forkhead; required for rRNA processing
Others
    ARC35Subunit of the ARP2/ARP3 complex, which is required for the motility and integrity of cortical actin patches
    IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase); catalyzes an essential activation step in the isoprenoid biosynthetic pathway
    NAT2N-α-Acetyltransferase; transfers acetyl group from acetyl coenzyme A to the N-terminal methionine residues of proteins
    SIS1Type II HSP40 cochaperone that interacts with the HSP70 protein Ssa1p
    STS1Protein that interacts with the karyopherin Srp1p; may have a role with Srp1p in ubiquitin-mediated protein degradation
    TOM40Component of the TOM (translocase of outer membrane) complex, responsible for recognition and initial import steps for all mitochondrially directed proteins