TABLE 4.

Genes involved in V-ATPase composition and assembly identified in this study as determinants of yeast resistance to inhibitory ethanol concentrationsa

Gene/ORF nameProtein description
CUP5Proteolipid subunit of the V-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit)
PPA1Subunit c″ of V-ATPase; one of three proteolipid subunits of the V0 domain
RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme
TFP3V-ATPase V0 domain subunit c′, involved in proton transport activity
THP2V-ATPase V0 domain subunit c′, involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen
VMA2Subunit B of the eight-subunit V1 peripheral membrane domain of the V-ATPase
VMA4Subunit E of the eight-subunit V1 peripheral membrane domain of the V-ATPase
VMA5Subunit C of the eight-subunit V1 peripheral membrane domain of V-ATPase
VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of V-ATPase
VMA8Subunit D of the eight-subunit V1 peripheral membrane domain of the V-ATPase
VMA9Subunit e of the V-ATPase V0 subcomplex
VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the V-ATPase
VMA21Integral membrane protein that is required for the assembly of V-ATPase
VMA22Integral membrane protein that is required for the assembly of V-ATPase