TABLE 3

Bacterial strains and plasmids used in this work

Strain or plasmidProperty(ies)aReference or source
Strains
    E. coli DH5αsupE44 ΔlacU169 (Ф80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA123
    E. coli BL21(DE3)F ompT hsdS B (rB mB) gal dcm λDE3Novagen/Merck (Darmstadt, Germany)
    E. coli T7SHuffle ExpressfhuA2 lacZ::T7 gene1 [lon] ompT ahpC gal λatt::pNEB3-r1-cDsbC (SpecR, lacIq) ΔtrxB sulA11 R(mcr-73::miniTn10-TetS)2 [dcm] R(zgb-210::Tn10-TetS) endA1 Δgor Δ(mcrC-mrr)114::IS10NEB (Frankfurt am Main, Germany)
    Deinococcus maricopensis DSM-21211Type strainDSMZ (Braunschweig, Germany)
    Vibrio gazogenes DSM-21264Type strainDSMZ (Braunschweig, Germany)
    Polyangium brachysporum DSM-7029Type strainDSMZ (Braunschweig, Germany)
Plasmids
    pET21a(+)Expression vector, lacI, Ampr, T7 lac promoter, C-terminal His6-tag coding sequenceNovagen/Merck (Darmstadt, Germany
    pET28a(+)Expression vector, lacI, Ampr, T7 lac promoter, C-terminal His6-tag and N-terminal coding sequenceNovagen/Merck (Darmstadt, Germany
    pEX-A2Cloning vector, Ampr, PlaclacZ, pUC oriEurofins MWG Operon (Ebersberg, Germany)
  • a Ampr, ampicillin resistance.