TABLE 3.

Numbers and percentages of fecal microorganisms from 11 volunteers as determined by DAPI staining (total cells) and by hybridization with bacterial probe Bact338 (total bacteria) or newly designed probes

PopulationStain or probeNo. of cells/g (dry wt) in volunteer no.a:
1234567891011
Total cellsDAPI1.4 × 10112.9 × 10113.2 × 10111.5 × 10111.4 × 10112.5 × 10112.0 × 10113.7 × 10112.4 × 10111.6 × 10118.6 × 1010
Total bacteriaBact 3387.9 × 10101.7 × 10112.2 × 10111.3 × 10116.2 × 10108.4 × 10101.7 × 10112.2 × 10111.2 × 10116.0 × 10108.3 × 1010
Ruminococcus groupRbro729/Rfla7302.0 × 109 (2.5)3.1 × 1010 (18.4)1.7 × 1010 (8.1)8.0 × 108 (0.6)1.7 × 109 (2.8)1.4 × 1010 (16.9)2.7 × 109 (1.6)4.4 × 1010 (19.8)3.5 × 1010 (28.4)6.6 × 109 (10.9)3.1 × 109 (3.8)
E. cylindroides groupEcyl3872.6 × 108 (0.3)2.0 × 108 (0.1)1.9 × 108 (0.09)9.3 × 109 (7.4)3.3 × 108 (0.5)3.4 × 109 (4.1)7.8 × 107 (0.05)1.3 × 108 (0.06)8.0 × 108 (0.6)8.5 × 108 (1.4)5.2 × 108 (0.6)
Phascolarctobacterium groupPhasco 7413.9 × 108 (0.5)1.5 × 108 (0.1)2.8 × 109 (1.3)2.1 × 108 (0.2)NDbND4.3 × 109 (2.6)1.0 × 107 (0.005)4.7 × 106 (0.004)7.4 × 106 (0.01)2.1 × 109 (2.5)
VeillonellaVeil2236.0 × 107 (0.07)ND6.1 × 106 (0.003)ND6.1 × 106 (0.01)3.4 × 107 (0.04)7.8 × 108 (0.5)3.7 × 107 (0.02)1.3 × 107 (0.01)6.0 × 107 (0.1)1.4 × 108 (0.2)
E. hallii groupEhal14695.7 × 109 (7.1)1.4 × 1010 (8.2)7.2 × 109 (3.4)7.1 × 109 (5.7)2.4 × 109 (3.9)8.8 × 108 (1.0)1.1 × 1010 (6.7)4.6 × 109 (2.0)1.3 × 109 (1.1)5.6 × 108 (0.9)1.4 × 109 (1.7)
Lachnospira groupLach5713.1 × 109 (3.9)7.6 × 109 (4.6)6.2 × 109 (2.9)2.1 × 109 (1.7)1.3 × 109 (2.1)1.3 × 109 (1.6)2.3 × 1010 (13.6)5.8 × 109 (2.6)2.8 × 109 (2.3)1.1 × 109 (1.8)2.3 × 109 (2.8)
% Dry matter of the fecal sample3036262537331724353322
  • a Values in parentheses represent the percentage relative to the total number of cells determined by hybridization with probe Bact338.

  • b ND, not detected.