TABLE 3.

Genes involved in lipid, fatty acid, and ergosterol biosynthesis identified in this study as determinants of yeast resistance to inhibitory ethanol concentrationsa

Gene/ORF nameProtein description
ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
ERG24C-14 sterol reductase, acting in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14-dienol)
IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase; catalyzes an essential activation step in the isoprenoid biosynthetic pathway
KCS1Inositol hexaphosphate kinase; phosphorylates inositol hexakisphosphate (InsP6) to diphosphoinositol polyphosphates, required for proper vacuole morphology
LIP5Protein involved in biosynthesis of the coenzyme lipoic acid
OPI3Phospholipid methyltransferase (methylene-fatty acyl-phospholipid synthase); catalyzes the last two steps in phosphatidylcholine biosynthesis
PDX3Pyridoxine (pyridoxamine) phosphate oxidase