TABLE 2.

Seven new probes, their target species, probe sequences, and optimal hybridization conditions for the detection of human fecal bacteria

ProbeTarget speciesProbe sequenceHybridization conditions
% of formamideLysozyme treatment (min)
Phasco741Phascolarctobacterium faecium, Acidaminococcus fermentans, and Succiniclasticum ruminis5′TCAGCGTCAGACACAGTC0NTa
Veil223Veillonella dispar, Veillonella parvula, and Veillonella atypica5′AGACGCAATCCCCTCCTT0NT
Ehal1469Eubacterium hallii, Clostridium herbivorans, and Clostridium polysaccharolyticum5′CCAGTTACCGGCTCCACC20
Lach571Lachnospira multipara, Eubacterium eligens, and Lachnospira pectinoschiza5′GCCACCTACACTCCCTTT40 (400 mM NaCl)NT
Ecyl387Eubacterium cylindroides, Clostridium innocuum, Eubacterium biforme, Eubacterium tortuosum, Eubacterium dolichum and Streptococcus pleomorphus5′CGCGGCATTGCTCGTTCA20NT
Rfla729Ruminococcus albus and Ruminococcus flavefaciens5′AAAGCCCAGTAAGCCGCC2015
Rbro730Clostridium sporosphaeroides, Ruminococcus bromii, and Clostridium leptum5′TAAAGCCCAGYAGGCCGC2015
  • a NT, no treatment.