TABLE 2.

Detection rates of bacterioplankton by FISH with HRP-labeled probes and CARD after 1.5 and 2.0 h of hybridization, respectively, and by FISH with Cy3-monolabeled probesa

Probe (hybridization period [h])LabelDetection rate (% DAPI)
MeanRange
EUB338 (1.5)HRP81.355.0-99.6
EUB338 (2.0)HRP93.884.9-100.0
EUB338Cy347.819.4-66.2
ROS537HRP4.71.9-7.2
ROS537Cy34.62.4-7.1
SAR86HRP7.42.9-13.2
SAR86Cy3NDb
NON338HRPND
  • a EUB338, most bacteria; SAR86-1249, members of the SAR86 clade; ROS537, members of the Roseobacter clade; NON338, EUB338 antisense probe.

  • b ND, not detectable (<1 positive cell in 10 microscopic fields).