TABLE 4.

Mean numbers per gram of feces, CVs, and percentages of total microbiota from 11 volunteersa

PopulationStain or probeMean no. of cells/g of feces (SD)bCVinterc% Microbiota by:
Dry wtWet wtDAPIBact338
Total cellsDAPI2.1 (0.8) × 10116.2 × 10100.38100
Total bacteriaBact3381.3 (0.6) × 10113.5 × 10100.4660.9100
Bacteroides/PrevotellaBac3033.6 (2.3) × 10109.5 × 1090.6218.427.7
E. rectale/C. coccoides groupErec4822.9 (1.9) × 10107.9 × 1090.6214.022.7
Eubacterium low G+C2Elgc011.4 (1.2) × 10103.6 × 1090.867.110.8
Atopobium groupAto2911.4 (0.9) × 10104.1 × 1090.676.911.9
Ruminococcus groupRbro729/Rfla7301.4 (1.6) × 10104.4 × 1091.075.510.3
BifidobacteriumBif1646.0 (4.0) × 1091.7 × 1090.612.74.8
E. cylindroides groupEcyl3871.5 (2.8) × 1094.0 × 1081.890.91.4
Phascolarctobacterium groupPhasco7419.0 (15) × 1082.0 × 1081.620.50.6
EnterobacteriaceaeEcoli15313.2 (9.1) × 1086.1 × 1072.840.20.2
VeillonellaVeil2231.0 (2.3) × 1082.1 × 1072.200.060.08
Lactobacillus/EnterococcusLab1581.2 (2.6) × 1074.1 × 1062.110.010.01
Sum of specific probes56.290.5
E. hallii groupEhal14695.1 (4.4) × 1091.4 × 1090.852.53.8
Lachnospira groupLach5715.1 (6.2) × 1091.3 × 1091.212.53.6
  • a Values represent microbiota from the same 11 volunteers in Table 3, as determined by DAPI staining and FISH with the extensive probe set for the predominant fecal microflora.

  • b Mean numbers from 11 fecal samples were calculated assuming that the numbers below the detection limit were zero.

  • c CVinter, CV due to normal differences in microbiota composition between the 11 human volunteers corrected for assay error.