TABLE 1.

Scaffoldins and dockerin-tagged cellulases used in this study

Protein nameDescription (from N terminus to C terminus)Host cellTag
Scaf-cScaffoldin containing a cohesin from C. cellulolytica followed by a CBDS. cerevisiaec-Myc
Scaf-ctScaffoldin containing a cohesin from C. cellulolytica followed by a CBD followed by a second cohesin from C. thermocellumS. cerevisiaec-Myc
Sacf-ctfScaffoldin containing a cohesin from C. cellulolytica followed by a CBD followed by a second cohesin from C. thermocellum and a third cohesin from R. flavefaciensS. cerevisiaec-Myc
AtEndoglucanase CelA from C. thermocellum fused with its native dockerinE. colic-His6
EcExoglucanase CelE from C. cellulolytica fused with its native dockerinE. colic-His6
GfEndoglucanase CelG from C. cellulolytica fused with a dockerin from R. flavefaciensE. colic-His6
BglAβ-Glucosidase BglA from C. thermocellum fused with a dockerin from R. flavefaciensE. colic-His6