TABLE 1.

Phenotypic and genomic data from characterized strains of roseobactersa

StrainSurface colonization/association (isolation source)MotilityChemotaxisQSInhibitory behaviorReference(s)
PGPGPGPG
Citricella sp. strain SE45+b (salt marsh grass)+c++d++e+12; R. N. Slightom and A. Buchan, unpublished data
Dinoroseobacter shibae DFL-12+ (dinoflagellate)+++++5
Loktanella vestfoldensis SKA53+ (microbial mat)+++88
Maritimibacter alkaliphilus HTCC2654Atlantic Ocean (Sargasso Sea) surface waters+++42
Pelagibaca bermudensis HTCC2601Atlantic Ocean (Sargasso Sea) surface waters+++16
Phaeobacter gallaeciensis BS107+ (scallop larva)++++++74, 75
Phaeobacter gallaeciensis 2.10+ (marine alga)+++++++69-71
Phaeobacter sp. strain Y4I+b (coastal Atlantic Ocean surface waters)+c++d+++e+R. N. Slightom and A. Buchan, unpublished data
Oceanibulbus indolifex HEL-45North Sea surface waters+++92
Oceanicola batensis HTCC2597Atlantic Ocean (Sargasso Sea) surface waters+15
Oceanicola granulosus HTCC2516Atlantic Ocean (Sargasso Sea) surface waters+++15
Roseobacter denitrificans OCh114+ (seaweed)+++8, 78
Roseobacter litoralis OCh149+ (seaweed)++++8, 78
Roseovarius nubinhibens ISMb (Caribbean Sea surface waters)+++8, 31; R. N. Slightom and A. Buchan, unpublished data
Roseovarius sp. strain TM1035− (dinoflagellate)+?+++++8
Sagittula stellata E-37+b (coastal Atlantic Ocean surface waters)++d++e+32; R. N. Slightom and A. Buchan, unpublished data
Silicibacter lacuscaerulensis ITI-1157+b (Icelandic geothermal lake)+d+e66; R. N. Slightom and A. Buchan, unpublished data
Silicibacter pomeroyi DSS-3+b (coastal Atlantic Ocean surface waters)++d++++8, 31, 59; R. N. Slightom and A. Buchan, unpublished data
Silicibacter sp. strain TM1040+ (dinoflagellate)++++++8, 56
Sulfitobacter sp. strain EE-36+ (coastal Atlantic Ocean surface waters)++d+8, 31; R. N. Slightom and A. Buchan, unpublished data
Sulfitobacter sp. strain NAS-14.1+b (Atlantic Ocean [Sargasso Sea] surface waters)+c+d+eR. N. Slightom and A. Buchan, unpublished data
% Correlation67886780
  • a “P” columns show phenotypic data; the absence of a sign indicates that no data are available. “G” columns show genomic data: the presence (+) or absence (−) of genes coding for flagella (motility), chemotaxis, QS systems, and NRPS and/or PKS (antagonistic behavior) as described in Table S1 in the supplemental material. Insufficient evidence for conclusive determination is indicated with a question mark.

  • b Surface colonization of these strains was determined by measuring biomass adherent to polystyrene and polyvinyl chloride dishes after 13 h of incubation at 30°C using a crystal violet assay (63). + and − indicate average optical densities at 590 nm (from at least 24 trials) of >0.5 and <0.3, respectively.

  • c Motility of these strains was determined using standard semisolid agar assays and confirmed by visualization of flagella in electron micrographs.

  • d Qualitative evidence for chemotaxis in these strains was determined by the development of concentric bands of cells in semisolid agar media per the description of Adler (1).

  • e Evidence of inhibitory behavior of these strains against Vibrio fischeri was determined using a well diffusion assay (37). A plus sign indicates the presence of a zone of inhibition around the test culture after 24 h.