TABLE 5.

Pathotypes, serotypes, and allelic and MLST profiles of E. coli O157 and E. coli K-12 strains

Pathotype or groupSerotypeNo. of strainsMLST profileMLST result for:XbaI PFGE groupa
adkarcAfumCmdhmtlD
EHECO157:H7b2I11111
O157:H76I11111B
O157:H71I11111C
O157:NM3I11111C
O157:H71I11111
EPECO157:H711I11111A
AtypicalO157:H121II23223
O157(rough):H12c1III23224
O157:H433IV34335D
O157(rough):H37rc1V34336D
O157(rough):H9c1V34336
O157:NM1V34336
O157(rough):H12c1VI44421
Nonpathogenic strainsK-122VII22222
  • a PFGE groups A to D are listed. Five atypical (eae- and stx-negative) O157 strains and the epidemiologically unrelated bovine E. coli O157 strain 4979 did not establish a further PFGE group.

  • b Two EHEC prototype strains (EDL933 and Sakai) and two epidemiologically unrelated strains (4979 of bovine origin and C81 [53] of human origin) were used in PFGE studies (Fig. 3). The allelic profile of C81 was not investigated.

  • c Identified as O157 and rfbE positive by PCR and by latex agglutination and as O rough by tube agglutination.