TABLE 3.

Phenotypic characterization of P. putida A isolates belonging to the genotypic clusters defined in Fig. 2

Genotype groupaStrainOriginActivity againstb:Lytic enzyme productioncSecondary metabolite productionAHL productionPhenotype group
V. dahliaeR. solaniS. sclerotiorumP. cactorumGlucanaseChitinaseProteaseKing BdDAPGeHCNfIAAgMT 102hF117i
G18Kz4PotatoCellulose++++++++++2
8Kz8PotatoCellulose+++++++++2
4Kc15PotatoChitin+++++++++1
G29Rp10/2RapeCasein++++++++5
G32Rr14RapeR2A++++++++5
8Rr24RapeR2A+++++++++3
G49Rz12/2RapeCellulose+++5
9Rz10/2RapeCellulose+++5
9Rc9RapeChitin++++++++++7
9Rp2RapeCasein++++7
9Rp10RapeCasein++++5
G58Kr32PotatoR2A+++++ND7
G67Ec5StrawberryChitin++++++ND6
G77Bp13/2SoilCasein+++++++++5
G87Ez9/2StrawberryCellulose+++
10Er28StrawberryR2A+++++++++1
G92Bx14SoilXylan+++++++++1
9Bc2SoilChitin++++++++++3
G104Kc13PotatoChitin++++++++++1
10Kx10/2PotatoXylan+++++++++1
G119Bp8SoilCasein+++++++++2
10Bp12SoilCasein++++++++++1
G129Bc8SoilChitin++++++++++++1
9Bc10SoilChitin++++++++++1
9Bc3SoilChitin++++++++++1
G132Bx4SoilXylan++++++++++1
2 Bx13SoilXylan+++++++++1
G149Rx5RapeXylan++++++++5
G159Rx7RapeXylan+++++++5
G169Rx24RapeXylan+++++++5
9Rx29RapeXylan+++++++++++5
G179Rx11RapeXylan+++++++5
G189Ex1/2StrawberryXylan++++++ND4
7Ez14/2StrawberryCellulose++++++6
7Ex1StrawberryXylan++++++ND6
  • a Grouping at 89% similarity

  • b Antagonism toward these four species was determined by dual-culture assay. Results indicate the width of the zone of inhibition as follows: +, 0 to 5 mm; ++, 5 to 10 mm; +++, 10 to 15 mm; ++++, >15 mm.

  • c β-1,3-Glucanase, protease, and chitinase activities were determined by plate assay (+, hydrolysis; −, no hydrolysis).

  • d Fluorescence on King's B agar.

  • e DAPG, 2,4-diacetylphloroglucinol; PCR approach.

  • f Production of cyanide as shown by the Merck Schnelltest: (+, > 0.001 mg liter−1).

  • g Microplate method of Sawar and Kremer (41) (+, > 0.1 μg ml−1).

  • h E. coli MT 102(pSB403) for detection of short-chain AHLs.

  • i P. putida F117(pKR-C12) for detection of long-chain AHLs.

  • j Grouping at 75% similarity.