TABLE 2.

Taxonomic classification and characterization of bacterial isolates with antagonistic properties belonging to Enterobacteriaceae

Genotype groupaStrainFAME analysis16S rDNA sequencingActivity againstb:Lytic enzyme productioncAHL production
IdentificationIDIdentification% Similarity (strain)V. dahliaeR. solaniS. sclerotiorumP. cactorumGlucanaseChitinasechiA productProteaseMT 102dFI17e
G14Rx13Serratia odorifera0.735Pantoea agglomerans99 (100-860)++++++++++
Pantoea agglomerans0.701
3Rp5Xenorhabdus luminescens0.014S. proteamaculans99 (DSM4543)++++++++++++
3Rr8Serratia plymuthica0.890++++++++++
3Rc15Serratia proteamaculans0.839S. proteamaculans99 (DSM4543)++++++++++++
3Rc3Serratia fonticola0.640S. plymuthica99 (DSM4540)+++++++++++++
4Rx5Serratia plymuthica0.715S. proteamaculans99 (DSM4543)+++++++++
4Rx10Salmonella enterica serovar Typhimurium0.405S. proteamaculans99 (DSM4543)+++++++++
4Rz11Pantoea agglomerans0.723S. proteamaculans99 (DSM4543)+++++++++++
4Rx4Xenorhabdus nematophilia0.146S. plymuthica99 (DSM4540)+++++++++
10Ep11Proteus vulgaris0.834S. plymuthica98 (124-822)+++++++++
3Rc14Pantoea agglomerans0.871
Klebsiella pneumoniae0.829++++++++++++
3Rr9Serratia proteamaculans0.793S. proteamaculans99 (DSM4543)++++++++++++
4Rr2Proteus vulgaris0.758+++++++++++++
Serratia grimesii0.706
4Rz1Enterobacter intermedius0.591++++++++++
2Kr27Kluyvera cryorescens0.383S. proteamaculans99 (DSM4543)++++
G24Rz6Serratia grimesii0.718++++++++++
Pantoea agglomerans0.714
3Rp8Serratia grimesii0.614+++++++++++++
Pantoea agglomerans0.589
9Ez29Serratia grimesii0.768+++++++++++
Pantoea agglomerans0.765
4Rr6Proteus vulgaris0.721+++++++++++
Pantoea agglomerans0.677
4Rc14Enterobacter intermedius0.736
0.675+++++++++
Serratia grimesii
9Ez25Serratia grimesii0.777+++++++
G39Ep9Salmonella enterica serovar Typhimurium0.496++++
9Rz10Serratia grimesii0.928+++++++++
5Rr4Weeksella zoohelcum0.560+++++++++
10Bp14Pantoea agglomerans0.884S. plymuthica99 (DSM4540)++++
8Rx9Weeksella zoohelcum0.478+++++
4Rc6Serratia plymuthica0.790++++++++++++
9Bp4Serratia proteamaculans0.740+++
3Bz10Serratia grimesii0.928+++
9Ec15Xenorhabdus nematophilia0.096++++
9Ez3Salmonella enterica serovar Typhimurium0.398+++
9Rz4Pantoea agglomerans0.89++++++++++++
Serratia grimesii0.872++++++++++++
  • a Grouping at 89% similarity.

  • b Antagonism toward these four species was determined by dual-culture assay. Results indicate the width of the zone of inhibition as follows: +, 0 to 5 mm; ++, 5 to 10 mm; +++, 10 to 15 mm; ++++, >15 mm.

  • c β-1,3-Glucanase, protease, and chitinase activities were demonstrated by plate assay (+, hydrolysis; −, no hydrolysis). The chiA gene was detected by a PCR approach.

  • d E. coli MT 102(pSB403) for detection of short-chain AHLs.

  • e P. putida F117(pKR-C12) for detection of long chain AHLs.