TABLE 1.

Bacterial and fungal strains used in this study

StrainDescriptionDH no.aSource or reference
Pseudomonas aeruginosa strains
    PA14 WTWT12341
    PA14 phzM::TnMTnM mutant, pyocyanin negative69328
    PA14 phzS::TnMTnM mutant, pyocyanin negative69828
    PA14 WT/pUCP26WT with empty plasmid from reference 5194251
    PA14 phzM::TnM/pUCP26Mutant with empty plasmid from reference 51944This study
    PA14 phzM::TnM/pUCP-MMutant complemented with the phzM gene on a plasmid (32)945This study
    PA14 phzS::TnM/pUCP26Mutant with empty plasmid from reference 51946This study
    PA14 phzS::TnM/pUCP-SMutant complemented with the phzS gene on a plasmid (32)947This study
    PA14 ΔphzIn-frame deletion mutant of phzA1 to phzG1 and phzA2 to phzG29339
    PA14 flgK::Tn5Tn5 mutant, nonmotile37
    PA14 pqsA::Tn5Tn5 mutant, lacks PQSThis study
    PAO1 WTWT2045
    PAO1 ΔphzMMutant lacking the phzM gene, pyocyanin negative29632
    PAO1 ΔphzSMutant lacking the phzS gene, pyocyanin negative29532
    Clinical isolatesIsolates from respiratory sputum211 to 228 and 74This study
Other Pseudomonas strains
    Pseudomonas fluorescens SWB25245G. O'Toole lab
    Pseudomonas putida KT2440468G. O'Toole lab
    Pseudomonas chlororaphis PCL1391469G. O'Toole lab
Fungal strains
    C. albicans SC5314WT6511
    C. albicans tup1/tup1 mutantBCa2-10; tup1/tup1 URA3/ura3362
    Saccharomyces cerevisiae Σ1278bΣ1278b347F. Winston lab
    S. cerevisiae BY4742MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0195F. Winston lab
    S. cerevisiae BY4741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0196F. Winston lab
    Environmental yeast isolate121This study
Escherichia coli strains
    DH5α/pUCP-M32
    DH5α/pUCP2632
  • a From our lab collection.