TABLE 2.

Purification of aerobic azoreductase from X. azovorans KF46F

Purification stepTotal protein (mg)Sp act (U/mg)Total activity (U)Recovery (%)Purifi- cation (fold)
Crude extract4020.02710.91001
Red Sepharose CL-6B111.1012.211141
Octyl-Sepharose + ultrafiltration0.74.53.330165
Gel filtration0.0910.81.09400