TABLE 4.

Closest cultured relatives of representative clones from the archaeal and bacterial 16S rRNA gene libraries

Type sequence from hydrate sedimentaNo. of clones in librarybClosest cultured relative
Above HZWithin HZBelow HZ
42 clones35 clones49 clones126Archaea
MA-C1-231Thermococcus celer
MA-C1-43Thermococcus celer
MA-C1-501Thermococcus celer
MA-A1-336Methanothermobacter thermophilus
MA-A1-16Desulfurococcus mobilis
MA-B1-38Desulfurococcus mobilis
MA-B1-527Desulfurococcus mobilis
MA-C1-34Desulfurococcus mobilis
MA-C1-510Pyrodictium occultum
37 clones50 clones34 clones121Bacteria
MB-B2-1052Unknown
MB-A2-1074Prevotella pallens
MB-A2-1181Prevotella tannerae
MB-C2-1051Pirellula sp.
MB-C2-1472Pirellula sp.
MB-C2-1281Duganella zoogloeoides
MB-A2-1151Kingella denitrificans
MB-A2-1022Curacaobacter baltica
MB-A2-1491Idiomarina loihiensis
MB-A2-1064Pseudomonas fluorescens
MB-A2-1371Pelobacter acetylenicus
MB-B2-1069Pelobacter acetylenicus
MB-C2-1521Pelobacter acetylenicus
MB-A2-1012Dehalococcoides ethenogenes
MB-C2-1274Dehalococcoides ethenogenes
MB-A2-1032Dehalococcoides ethenogenes
MB-C2-1261Dehalococcoides ethenogenes
MB-A2-1101Dehalococcoides ethenogenes
MB-B2-1132Dehalococcoides ethenogenes
MB-A2-1005Acidimicrobium ferrooxidans
MB-A2-1084Frankiamyricanod
MB-B2-10723Streptomyces thermodiastaticus
MB-C2-12016Streptomyces thermodiastaticus
MB-A2-1046Moorella glycerini
MB-B2-10311Moorella glycerini
MB-C2-1061Moorella glycerini
MB-A2-1053Moorella glycerini
MB-B2-1163Moorella glycerini
MB-C2-1037Moorella glycerini
  • a Type sequences are sequences from representative clones having unique RFLP patterns. Above HZ, above hydrate stability zone at 165.5 mbsf; within HZ, within the hydrate stability zone at 248.4 mbsf; below HZ, below the hydrate stability zone at 297.6 mbsf.

  • b Number of clones represented by sequence data.