TABLE 3.

Phylogenetic identification and pure culture statistics for 56 cultures

Inoculation date (mo-day-yr)No. of wells screenedNo. of cultures detectedTheoretical no. of pure culturesaNo. with culture identification
SAR11OM43SAR92OM60/OM241OthercMixed cultureNot identified
5-21-989676.7 (2.8, 12.8)52
6-17-98961110.3 (5.5, 16.8)21423b
10-29-9996109.5 (4.8, 15.9)811
1-26-001921110.7 (5.5, 18.2)7211
7-12-001441716.0 (9.8, 23.7)33812
Total6245653.4 (41.2, 67.4)2161531182
  • a Statistical estimation of the theoretical number of pure cultures acquired with 95% confidence interval. The total 53.4 was determined independently with 624 wells and 56 cultures in the pure culture equation.

  • b One SAR11 culture was mixed with an unknown cell type (RFLP analysis) and is also included under the heading “Mixed culture.”

  • c “Other” indicates cultures that fall into previously cultured groups.