TABLE 1.

PCR primers used in the present study

GenePrimerSequence (5′ → 3′)aDerivation sequenceOrganism (GenBank accession no.)Reference for the derivation sequence
16S rRNAEUB338-FACT CCT ACG GGA GGC AGCN338-N355Escherichia coli (7415865)43
RM2-RGAT CTC TAC GCA TTT CAC CGC TACN685-N708
nirSKA3-FCAC GGY GTB CTG CGC AAG GGC GCN292-N314Paracoccus denitrificans (U05002)10
KA25-RCGC CAC GCG CGG YTC SGG GTG GTAN967-N990
nirKK15-FGGC ATG GTA CCT TGG CAC GTA ACC TCG GGCN526-N555Alcaligenes faecalis (D13155)28
K16-RCAT TAG ATC GTC GTT CCA ATC ACC GGTN1075-N1101
nosZnosZ-FCGY TGT TCM TCG ACA GCC AGN1211-N1230Paracoccus denitrificans (398932)13
nosZ-RCAT GTG CAG NGC RTG GCA GAAN1897-N1917
nifHnifH-FAAA GGY GGW ATC GGY AAR TCC ACC ACN34-N59Sinorhizobium meliloti (46285)40
nifH-R orTTG TTS GCS GCR TAC ATS GCC ATC ATN466-N491
K07-FGCG TTC TAC GGT AAG GGC GGT ATC GGN AARN22-N51
AMR-RGCT ACT ACY TCG CCS GAN457-N473
  • a Boldface letters denote degenerate positions. B, G + T; M, A + C; N, A + C + G + T; R, A + G; S, G + C; Y, C + T; W, A + T.