TABLE 2.

The occurrence of genes coding for denitrification enzymes and nitrogenase reductase in laboratory cultures assessed by PCR amplification

Bacterial strainPCR result for indicated gene (primer pair)
nirS (KA3-KA25)nirK (K15-K16)nosZ (nosZ-F-nosZ-R)nifH (nifH-F-nifH-R)nifH (K07-AMR)
Proteobacteria, α subdivision
    Azospirillum amazonense Y-1T++
Azospirillum brasilense Sp7030 (nirS mutant)+++
Azospirillum brasilense Sp7T++++
Azospirillum doebereinerae GSF 71T++++
Azospirillum halopraeferens Au4T++++
Azospirillum irakense KA3++++
Azospirillum irakense KBCIT+++
Azospirillum largomobile ACM 2041+++
Azospirillum lipoferum Sp59bT++++
Azospirillum strain SpA1-3++++
Azorhizobium caulinodans ORS 571T+++
Blastobacter denitrificans IFAM 1005T
Bradyrhizobium japonicum LMG 6137++
Gluconacetobacter diazotrophicus PAL3+++
Ochrobactrum anthropi LMG 3331++
Paracoccus denitrificans Pd1222++
Rhizobium trifolii ANV 845++
Rhizobium tropici CIAT 899+++
Rhodospirillum rubrum S1T+++
Sinorhizobium meliloti Rm1021++++
Proteobacteria, β subdivision
Achromobacter ruhlandii DSM 653T+
Achromobacter xylosoxidans NCIMB 11015+
Aquaspirillum autotrophicum SA32T+++
Burkholderia brasilensis” M130++
Herbaspirillum frisingense GSF 30T++++
Herbaspirillum rubrisubalbicans LMG 1278+++
Herbaspirillum seropedicae Z67T++++
Herbaspirillum seropedicae Z78+++
Ralstonia eutropha H16+++
Proteobacteria, γ subdivision
Azomonas agilis DSM 375+++
Azorhizophilus paspali DSM 2283++
Azotobacter vinelandii DSM 2289++
Enterobacter cloacae NCIMB 11463++
Escherichia coli K-12
Frateuria aurantia DSM 6220T+++
Pseudomonas aeruginosa DSM 6195T++
Pseudomonas chlororaphis NCIMB 9030
Pseudomonas stutzeri (= Alcaligenes faecalis) A15++++
Pseudomonas stutzeri ZoBell 632+++
Firmicutes, Actinomycetales
Frankia alni ArI3 (DSM 44251)++
Frankia strain DSM 43829++
Frankia strain DSM 44263++
Kocuria varians LTH 1429
Micrococcus luteus DSM 20030T
Nocardioides simplex DSM 20130T
Rhodococcus erythropolis (from H. Papen, Garmisch-    Partenkirchen)+
Firmicutes, Bacillus/Clostridium group
Clostridium pasteurianum DSM 525T++
Paenibacillus validus DSM 3037T++
Staphylococcus carnosus LTH 55
Cyanobacteria
Anabaena variabilis ATCC 29413+
Chroococcidiopsis thermalis” PCC 7203+
Microcystis strain PCC 7806
Nostoc muscorum Agardh CCAP 1453/12+
Plectonema boryanum PCC 6306+
Synechococcus strain PCC 7942
  • a +, PCR product of the right size obtained; −, no PCR product obtained; boldface +, PCR product was cloned and sequenced and the sequence was deposited in the data banks.