TABLE 1.

Extinction culturability statistics compared to traditional culturability counts

Date (mo-day-yr) and location of inoculation sampleaInoculum sample (cells/ml)Avg no. of cells/wellTotal no. of wells inoculatedNo. of positive wellsbCulture designations% Culturabilityc% Culturability on nutrient-rich agard
1/10R2AR2AMA2216
5-21-98, J1.1 × 1061.11447HTCC1-74.5 (1.8, 9.3)
6-5-98, J1.5 × 1061.519237HTCC8-4414.3 (10.0, 19.7)
7-6-98, 8 km3.7 × 1063.719262HTCC45-10610.5 (8.0, 13.5)
7-6-98, 25 km1.5 × 1061.519237HTCC107-14314.3 (10.0, 19.7)
6-17-99, J5.6 × 1063.019221HTCC144-1643.9 (2.4, 5.9)
10-29-99, J1.9 × 1063.019210HTCC165-1741.8 (0.9, 3.3)
12-21-99, J8.1 × 1055.038410HTCC175-1840.5 (0.3, 1.0)
1-26-00, J1.1 × 1065.019211HTCC185-191, 193-1961.2 (0.6, 2.1)0.010.010.02
4-5-00, J9.0 × 1055.019220HTCC197-2162.2 (1.3, 3.4)0.150.12
7-12-00, J1.9 × 1063.022833HTCC217-233, 236-2515.2 (3.6, 7.3)0.980.150.12
10-9-00, 8 km1.3 × 1063.03845HTCC252-2560.4 (0.1, 1.0)0.290.090.02
  • a Samples were collected on the date indicated from the jetty (J) or 8 or 25 km out from the mouth of Yaquina Bay, Oreg.

  • b Wells were scored for growth after 3 weeks of incubation at 16°C.

  • c Ninety-five percent confidence intervals are shown in parentheses.

  • d Inoculum was the same as that used for the microtiter plates. —, not determined.