TABLE 6.

Ratios of qPCR-derived copy number estimates of genes from M. avium subsp. paratuberculosis type II and type III strains

StrainRatio (n-fold) of copy no.a
Pre-16S rRNA geneMAP3746MAP2729MAP0859cMAP0101MAP0160
Type III strains
    8412.12.521.871.881.090.92
    CAM86 large colony4.091.91.511.350.821.28
    CAM86 small colony3.270.870.660.610.751.49
    CAM871.651.410.520.860.781.39
Type II strains
    456 large colony0.931.060.710.740.881.15
    456 small colony1.321.120.671.011NTb
    CAM63 large colony111111
    CAM63 small colony0.9111.311.11NT
    Vaccine II1.091.245.161.651.020.98
    K-10111111
  • a Significance between M. avium subsp. paratuberculosis type II and type III strains for the pre-16S rRNA gene and MAP3746 showed a P value of 0.037; all other values were nonsignificant.

  • b NT, not tested.