TABLE 1.

Temperature- and time-dependent total mcrA transcript and gene abundances, total DNA and RNA yields, and mcrA transcript and gene detection limits

ParameterMean (SE) per exptl data set for:
Peat stored for 4 mo (n = 15)Peat stored for 6 mo (n = 21)Effect-of-temp expt (n = 16)
mcrA transcript abundance/ng nucleic acid6.6 × 103 (1.7 × 103)3.9 × 102 (8.5 × 101)6.1 × 102 (9.7 × 101)
mcrA transcript abundance/g soil6.7 × 107 (1.2 × 107)1.1 × 107 (3.9 × 106)8.5 × 106 (1.4 × 106)
mcrA gene abundance/ng nucleic acid4.5 × 103 (4.3 × 102)2.4 × 103 (2.9 × 102)3.3 × 102 (4.6 × 101)
mcrA gene abundance/g soil1.7 × 108 (4.2 × 107)3.6 × 107 (3.9 × 106)3.4 × 107 (3.9 × 106)
Internal-standard-corrected mcrA transcript abundance/g soil4.8 × 108 (8.1 × 107)
Internal-standard-corrected mcrA gene abundance/g soil9.1 × 108 (1.5 × 108)
Detection limit for mcrA transcript abundance/g soil1.9 × 1036.5 × 1033.1 × 103
Detection limit for mcrA gene abundance/g soil3.2 × 1041.5 × 1041.5 × 105
Total DNA yield (μg/g)32 (2.0)15 (0.7)152 (12.0)
Total RNA yield (μg/g)26 (0.8)14 (0.8)17 (1.0)