TABLE 4.

Amplification efficiency in TNA for quantification of Synechococcus sp. strain BO 8807 in environmental samples

Date of sampling (day/mo/yr)Synechococcus sp. strain BO 8807 (genomes/ml)Amplification efficiencya
18/05/199912,640 ± 1,9640.974 ± 0.0045
02/06/19991,306 ± 2780.959 ± 0.0034
15/06/19991,483 ± 2480.966 ± 0.0038
29/06/1999379 ± 480.961 ± 0.0024
13/07/19992,725 ± 9250.976 ± 0.0032
03/08/1999144 ± 910.992 ± 0.0133
17/08/199994 ± 340.973 ± 0.0
31/08/1999147 ± 90.969 ± 0.0025
14/09/199920 ± 160.969 ± 0.0032
28/09/1999164 ± 160.972 ± 0.0110
12/10/1999840 ± 910.964 ± 0.0031
16/05/2000248 ± 730.954 ± 0.0095
30/05/2000203 ± 500.967 ± 0.0008
14/06/2000149 ± 230.999 ± 0.0
27/06/2000165 ± 1030.966 ± 0.0019
11/07/2000341 ± 1740.964 ± 0.0039
01/08/2000125 ± 740.970 ± 0.0033
15/08/200016 ± 130.984 ± 0.0278
29/08/2000624 ± 4310.962 ± 0.0030
12/09/2000105 ± 350.965 ± 0.0025
10/10/2000119 ± 520.970 ± 0.0004
  • a Defined by describing the yield y of PCR cycle n as yn + 1 = yn (1 + ε).