TABLE 2.

Calculation of lysogenic fractions of bacterial populations in Tampa Bay, Fla.a

Samples and date (day/mo/yr)No. of induced prophage ml−1Lysogenic fraction (%) determined by:Induced burst size (ΔVDC/ΔBDC)
Assuming BZ = 50%Assuming BZ = 30%Mortality methodb
Unfiltered
    2/9/004.60 × 107 ± 9.50 × 10643.6 ± 30.772.6 ± 51.224.0 ± 52.242 ± 218.7
    2/23/006.16 × 107 ± 1.34 × 10760.4 ± 52.3101.0 ± 87.212.6 ± 53.975.1 ± 252.3
    4/17/009.22 × 106 ± 5.69 × 1057.7 ± 2.312.8 ± 3.851.2 ± 36.94.8 ± 5.6
    8/7/002.53 × 107 ± 3.18 × 10612.8 ± 2.821.4 ± 4.623.3 ± 36.512.6 ± 3.7
    9/11/002.26 × 107 ± 1.22 × 10710.8 ± 5.918.1 ± 9.813.2 ± 36.713.3 ± 19.8
Virus reduced
    11/17/9916.7 × 106 ± 4.10 × 1051.4 ± 1.02.3 ± 1.66.4 ± 44.02.0 ± 5.3
    1/5/001.47 × 108 ± 6.43 × 107126.0 ± 113.1210.0 ± 188.433.4 ± 137.9102.8 ± 132.5
    1/18/002.32 × 108 ± 6.05 × 107103.8 ± 119.0172.9 ± 198.847.6 ± 114.468.8 ± 165.8
    2/9/001.50 × 107 ± 7.82 × 10612.6 ± 13.821.0 ± 23.238.8 ± 113.99.4 ± 44.0
    2/23/004.10 × 107 ± 2.89 × 10727.4 ± 22.345.7 ± 37.234.6 ± 36.522 ± 19.6
    5/16/004.80 × 106 ± 2.08 × 1062.6 ± 1.14.3 ± 1.926.5 ± 38.32.4 ± 1.7
    6/14/004.41 × 107 ± 3.44 × 10610.8 ± 0.618.1 ± 1.035.1 ± 38.08.6 ± 2.0
    7/17/001.87 × 106 ± 2.72 × 106.92 ± 0.71.5 ± 1.15.7 ± 45.61.4 ± 8.6
    10/23/005.82 × 106 ± 6.62 × 10636.4 ± 26.060.8 ± 43.34.1 ± 58.157.6 ± 80.1
Virus reduced (10 μM phosphate)
    8/21/004.80 × 106 ± 9.80 × 1052.0 ± 0.73.4 ± 1.26.8 ± 39.02.9 ± 3.3
    9/25/001.55 × 107 ± 4.91 × 1067.9 ± 2.913.2 ± 4.8≤0 ± 53.240.8 ± 18.0
        Avg4.00 × 107 ± 1.34 × 10727.6 ± 24.745.9 ± 41.221.6 ± 58.427.6 ± 61.3
  • a The lysogenic fraction of bacterial populations, the number of induced prophage per milliter, and the induced burst size were calculated for all unaltered, virus- reduced, and phosphate-treated samples that displayed statistically significant (90 to 95% confidence interval) increases in viral direct counts relative to control samples. Results are means ± standard deviations.

  • b Mortality caused by mitomycin C in uninduced samples = 27.6% ± 36.2%.