TABLE 2.

Metabolism of benzoate and fluorobenzoate isomers by pure cultures of S. aciditrophicus grown with crotonatea

Substrate(s)Maximum OD600bCrotonate used (mM)Cosubstrate used (mM)Acetate formed (mM)CHAC formed (mM)Fluoride formed (mM)Benzoate formed (mM)Final H2 (Pa)
Crotonate0.24 ± 0.017.1 ± 0.2NA10.4 ± 2.11.16 ± 0.08NA0.04 ± 0.019.4 ± 1.2
Crotonate + benzoate0.25 ± 0.017.7 ± 0.41.79 ± 0.1215.1 ± 0.91.67 ± 0.26NANA8.9 ± 2.3
Crotonate + 2-fluorobenzoate0.09 ± 0.012.2 ± 0.50.06 ± 0.032.2 ± 2.00.17 ± 0.010.06 ± 0.010.04 ± 0.0187.4 ± 7.8
Crotonate + 3-fluorobenzoate0.08 ± 0.013.7 ± 0.60.13 ± 0.025.7 ± 0.30.53 ± 0.140.15 ± 0.020.17 ± 0.01116.6 ± 13.9
Crotonate + 4-fluorobenzoate0.25 ± 0.016.7 ± 1.00.01 ± 0.029.9 ± 1.81.3 ± 0.200.005 ± 0.140.04 ± 0.017.8 ± 0.8
  • a Abbreviations: OD600, optical density at 600 nm; CHAC, cyclohexane carboxylate; NA, not applicable. The values are the averages ± standard deviations of triplicate cultures measured after 10 days of incubation.

  • b The maximum absorbance was reached within 6 days for the cultures grown in the presence of crotonate alone or crotonate and 4-fluorobenzoate; it was reached within 10 days for the cultures grown with crotonate and benzoate, 2-fluorobenzoate, or 3-fluorobenzoate.