TABLE 1.

Primer nucleotide sequences used to PCR amplify 10 viral groups

Human virusesPrimerSequence (5′-3′)bSensitivity (no. of targets)Amplicon size (bp)
AdenovirusesAV-A1GCC GCA GTG GTC TTA CAT GCA CAT C100300
AV-A2CAG CAC GCC GCG GAT GTC AAA GT
AV-B1aGCC ACC GAG ACG TAC TTC AGC CTG143
AV-B2aTTG TAC GAG TAC GCG GTA TCC TCG CGG TC
EnterovirusesJP UPTTA AAA CAG CCT GTG GGT TG100600
ENT DOWNACC GGA TGG CCA ATC
ENT UPaCCT CCG CCC CTG AAT G154
JP DOWNaATT GTC ACC ATA AGC GAC C
Hepatitis B virusesHBS-1ATC AGG ATT CCT AGG ACC C10,000310
HBS-R1AGG ACA AAC GGG CAA CAA C
HBS-11aGCG GGG TTT TTC TTG TTG AC241
HBS-R11aGAA CCA ACA AGA AGA TGA GGC
HerpesvirusesFP1GAY TTY GCI AGY YTI TAY CC10800
FP2TCC TGG ACA AGC AGC ARI YSG CIM TIA A
RP1GTC TTG CTC ACC AGI TCI ACI CCY TT
FP3aTGT AAC TCG GTG TAY GGI TTY ACI GGI GT215-235
RP2aCAC AGA GTC CGT RTC ICC RTA IAT
MorbillivirusesUpATG TTT ATG ATC ACA GCG GT100429
DownATT GGG TTG CAC CAC TTG TC
NorovirusesP290GAT TAC TCC AAG TGG GAC TCC AC10,000219
P289TGA CAA TGT AAT CAT CAC CAT A
PapillomavirusesFAP59TAA CWG TIG GIC AYC CWT ATT10,000478
FAP64CCW ATA TCW VHC ATI TCI CCA TC
Picobirnaviruses (genotype I)PicoB25TGG TGT GGA TGT TTC1200
PicoB43ART GYT GGT CGA ACT T
ReovirusesL1.rv5GCA TCC ATT GTA AAT GAC GAG TCT G100416
L1.rv6CTT GAG ATT AGC TCT AGC ATC TTC TG
L1.rv7aGCT AGG CCG ATA TCG GGA ATG CAG344
L1.rv8aGTC TCA CTA TTC ACC TTA CCA GCA G
Rotaviruses (group A)RV1GTC ACA TCA TAC AAT TCT AAT CTA AG1,0001,059
RV2CTT TAA AAG AGA GAA TTT CCG TCT G
RV3aTGT ATG GTA TTG AAT ATA CCA C346
RV4aACT GAT CCT GTT GGC CAW CC
  • a Internal primer set for a nested PCR.

  • b H equals A/C/T, M equals A/C, R equals G/A, S equals G/C, V equals G/C/A, W equals A/T, and Y equals T/G. Note that inosine (I) has base-pairing bias for C, A, G, and then T.