TABLE 1.

Sequence similarities to closest relatives and phylogenetic affiliations of DNA recovered from DGGE gel

BandaClosest relative
No.Accession no.Similarity (%)OrganismAccession no.Phylogenetic affiliationSource
1-1AY07187197Uncultured bacterium clone Ebpr10AF255634CFB groupBiological phosphorus removal process
1-2AY07187292Uncultured bacterium clone SR-425-3AF203939CFB groupPolluted groundwater
1-3AY07187386Uncultured bacterium clone SR-615-17AF203967CFB groupPolluted groundwater
2-1AY07187499Uncultured α-proteobacterium FL13A04AF446303α-ProteobacteriaYellowstone hot springs
2-2AY07187599Uncultured Cytophagales clone PRD01a001BAF289149CFB groupFreshwater
3-1AY07187698Uncultured β-proteobacterium ESR 9AF268293β-ProteobacteriaFreshwater
3-2AY071877100Caulobacter sp. strain A1AF361188α-ProteobacteriaFreshwater
3-3AY071878100Uncultured Crater Lake bacterium CL500-17AF316660High-G + C-content gram-positive bacteriaLake
4-1AY07187999Microbacterium foliorumAJ249780High-G + C-content gram-positive bacteriaPhyllosphere
4-2AY07188097Uncultured Crater Lake bacterium CL500-6AF316790CFB groupLake
5-1AY071881100Uncultured Crater Lake bacterium CL500-95AF316665High-G+C-content gram-positive bacteriaLake
5-2AY07188289Uncultured sludge bacterium A12bAF234699UnknownNitrifying-denitrifying activated sludge
5-3AY07188390Unidentified eubacterium clone BSV07AJ229181Low-G+C-content gram-positive bacteriaPaddy soil
6-1AY07188490Unidentified eubacteriumAJ005996CFB groupSoil
6-2AY07188596Uncultured eubacterium clone IAFR119AF270947High-G+C-content gram-positive bacteriaSoil
7-1AY07188697Flavobacterium columnareAB015480CFB groupDiseased fish
7-2AY07188798Uncultured firmicute clone GOBB3-CL124AF388884FirmicutesEnvironmental samples
7-3AY07188899Flavobacterium sp. strain GOBB3-209AF321038CFB groupEstuary
8-1AY07188990Uncultured bacterium clone TDC-S1:26AF447142CFB groupDegradation of tetrachloroethene
8-2AY07189098Uncultured Crater Lake bacterium CL120-17AF316668High-G+C-content gram-positive bacteriaLake
9-1AY07189187Uncultured CFB group bacterium clone CD13H9AF441869CFB groupSouthern Caribbean Sea
9-2AY071892100Uncultured Crater Lake bacterium CL0-27AF316644High-G+C-content gram-positive bacteriaLake
9-3AY071893100Uncultured soil bacterium C0111AF128708α-ProteobacteriaSoil
10-1AY07189494Unidentified gram-positive low-G + C-content bacteriumAJ239992Low-G+C-content gram-positive bacteriaSulfurous lakes
10-2AY07189598Uncultured bacterium SY6-50AF296201CFB groupLake
10-3AY07189695Uncultured Crater Lake bacterium CL0-93AF316708CFB groupLake
11-1AY07189799Uncultured Crater Lake bacterium CL500-67AF316664High-G+C-content gram-positive bacteriaLake
11-2AY07189897Uncultured bacterium clone Ebpr7AF255646CFB groupBiological phosphorus removal process
12AY07189998Bacterial 16S rRNA gene of isolate LD12Z99997α-ProteobacteriaCosmopolitan freshwater bacteria
13-1AY07190089Uncultured bacterium clone LO13.22AF358015CFB groupSoil
13-2AY07190195Uncultured Crater Lake bacterium CL120-125AF316675High-G+C-content gram-positive bacteriaLake
14AY07190299Uncultured Crater Lake bacterium CL500-91AF316679High-G+C-content gram-positive bacteriaLake
  • a Most of the bands consisted of multiple sequences, and representative sequences in each clone library are shown.