TABLE 2.

PCR primers used to characterize isolates from fecal samples and commercial insecticides

Target(s) and primer pairPrimer sequence (5′ to 3′)Amplicon size(s) (bp)Reference
Insecticidal genes
    Cry1aCTG-GAT-TTA-CAG-GTG-GGG-ATA-T543-59410
    Cry1bTGA-GTC-GCT-TCG-CAT-ATT-TGA-CT
    Cry3aTTA-ACC-GTT-TTC-GCA-GAG-A652-71812
    Cry3bTCC-GCA-CTT-CTA-TGT-GTC-CAA-G
    Cry11aTTA-GAA-GAT-ACG-CCA-GAT-CAA-GC30510
    Cry11bCAT-TTG-TAC-TTG-AAG-TTG-TAA-TCC-C
    CytaAAC-CCC-TCA-ATC-AAC-AGC-AAG-G522-52510
    CytbGGT-ACA-CAA-TAG-ATA-ACG-CCA-CC
16S-23S spacer region
    G1GAA-GTC-GTA-ACA-AGG240, 460, 530, 65013
    G2CAA-GGC-ATC-CAC-CGT
23S internal fragment
    GP1GGC-TCG-TCC-GCT-CAG-GG50836
    GP2AAC-CTT-CCA-GCA-CCG-GG
Phospholipase C
    plc1TGA-ATG-GCG-TAC-GGA-GTT-AGA415This study
    plc2CCG-CTC-CAT-GAA-TCC-ACT-C
Enterotoxin gene
    NheA1SATT-AAG-GTA-AAT-GCG-ATG-AG671This study
    NheA1AGCT-TCA-GTT-TGT-GAT-AAC-TT
    HblA3ACG-AAC-AAT-GGA-GAT-ACG-GC1,01746
    HblA4ATT-TTT-GTG-GAG-TAA-CAG-TTT-CTA-C
    BceT3TTA-CAT-TAC-CAG-GAC-GTG-CTT42746
    BceT4TGT-TTG-TGA-TTG-TAA-TTC-AGG