TABLE 1.

Purification of FpdA2 from E. coli BL21(DE3)(pET17b)

StepTotal vol (ml)Total protein (mg)Total enzyme activitya (U)Sp act (U mg−1)Yield (%)Purification (fold)
Cell extract381,3509.50.0071001
DEAE Sepharose152507.50.030794.3
Hydroxyapatite7386.00.1606323
  • a Reaction mixtures contained 400 μM 4-FP, a suitable amount of FpdA2, and the components given in Materials and Methods.