TABLE 2.

Kinetic parameters of FpdBa

Fixed substratebVaried substrateKm (μM)kcat (s−1)kcat/Km (M−1 s−1)
FADNADH13 ± 233 ± 42.5 × 106
FMNNADH122 ± 1412 ± 21.0 × 105
NADHFMN10 ± 163 ± 36.6 × 106
NADHFAD6 ± 123 ± 23.6 × 106
  • a Values refer to the varied substrate and are means for triplicate experiments with standard deviations.

  • b FAD and FMN concentrations were fixed at 100 μM, and the NADH concentration was fixed at 300 μM.