TABLE 3.

Conversion of 4-substituted phenols by purified FpdA2 and FpdBa

SubstrateRate (μmol/min mg−1)Concn (μM)b
Substrate degradedAnion formedHydroquinone
4-FP46344312145
4-Chlorophenol3929227992
4-Bromophenol7254149298
4-Nitrophenol40303280150
Hydroquinone6413010c
  • a Reaction mixtures contained 600 μM substrate, 0.15 mg ml−1 of 4-FP monooxygenase, and the components described in Materials and Methods. Aromatics were analyzed by HPLC or GC at different times, and anions were analyzed by ion chromatography.

  • b After 20 min of incubation.

  • c Hydroxyhydroquinone detected by GC.