TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or referencea
Strains
    Escherichia coli DH5αrecA endA1 gyrA96 thi-1 hsdR17 supE44 Δlac U169(φ80 ΔlacZΔM15) deoR F λGibco-BRL
    Leuconostoc mesenteroides subsp. mesenteroides
        Y105Wild-type strain18
        Y110Wild-type strainCNCM I-1936a
        FR52Wild-type strain24
    Leuconostoc mesenteroides subsp. dextranicum DSM20484Wild-type strainDSMZb
    Leuconostoc cremoris LCWild-type industrial strainRhodia Food
    Lactococcus lactis
        IL1403Lab strain8
        MG1363Lab strain13
        RD230Wild-type industrial strainRhodia Food
    Lactobacillus sake 23KPlasmid cured3
    Listeria ivanovii BUG 497Lab strainPasteur Institute
    Pediococcus acidilactici P120Wild-type industrial strainRhodia Food
Plasmids
    pBSSKII+pBluescript SK II+ cloning vector, 2.96 kb, Apr lacZStratagene
    pGhost9:ISS1pWV01 derivative replicon23
    pFR181.8-kb cryptic plasmid from L. mesenteroides FR525
    pFBYC018pBSSKII+::1.8-kb HindIII pFR18, 4.8 kb, Apr Ems5
    pFBYC018EpFBYC018::1.1-kb BamHI pGhost9:ISS1 (Emr), 5.9 kb, Apr Emr5
    pFBYC18EpFBYC018E with 2.5-kb PvuII pBSSKII+ deleted, 3.5 kb, Emr5
    pTXL12.66-kb cryptic plasmid from L. mesenteroides Y1105
    pFBYC050pBSSKII+::2.66-kb SalI pTXL1, 5.62 kb, Apr EmsThis study
    pFBYC051pBSSKII+::2.66-kb SpeI pTXL1, 5.62 kb, Apr EmsThis study
    pFBYC052pBSSKII+::1.8-kb SspI pFR18, 4.8 kb, Apr EmsThis study
    pFBYC050EpFBYC050::1.1-kb BamHI pGhost9:ISS1, 7.72 kb, Apr EmrThis study
    pFBYC50EpFBYC050E with 2.5-kb PvuII fragment deleted, EmrThis study
    pFBYC065pFBYC050E with 312-kb ExoIIIc fragment deleted, Apr EmrThis study
    pFBYC060KpFBYC050E with 482-kb KpnI-KpnI fragment deleted, Apr EmrThis study
    pFBYC062SpFBYC050E with 1.4-kb SalI-SspI fragment deleted, Apr EmrThis study
    pFBYC068SpFBYC050E with 1.9-kb SalI-AflIII fragment deleted, Apr EmrThis study
    pFBYC051EpFBYC051::1.1-kb BamHI pGhost9:ISS1, 7.72 kb, Apr EmrThis study
    pFBYC06pRW5.6 with HindIII deleted4
    pFBYC069pFBYC051E::669-bp P59, dvnA fragment from pFBYC06This study
    pFBYC070pFBYC069::485-bp dvnA::mesY, mesI PCR productThis study
  • a CNCM, Collection Nationale de Cultures de Microorganisms, France.

  • b DSMZ, Deutsche Sammlung von Mikroorganismen and Zellkulturen, Germany.

  • c ExoIII, exonuclease III.