Bacterial strains and plasmids used in this work

Strain or plasmidRelevant characteristicsaSource or reference(s)
    A. baumannii
        9235, 8971, 8143, 8114, 8637, 7133, 7138, 8399, 9606, 7931, 9124, 9397Clinical isolates representing plasmid profiles A to K and W, respectively2, 22
        BM4420, BM4421, BM4422, BM4424, BM4427, BM4430, BM4432, BM4436, BM4439Clinical isolates representing RAPD types A to F, H, I, and K, respectively46
    E. coli
        DH5αUsed for recombinant DNA methodsGibco-BRL (Gaithersburg, Md.)
        TransforMax EC100Dpir+, used to clone R6K derivativesEpicentre (Madison, Wis.)
        CC118, SM10, and S17-1λpir, used to maintain and conjugate suicide plasmid pLOFKm10, 26
    pLOFKmSuicide plasmid harboring mini-Tn10Km; Ampr Kmr26
    pVK100Cosmid cloning vector; Kmr Tetr33
    pRK2073Conjugation helper plasmid37
    pWH1266E. coli-Acinetobacter shuttle plasmid; Ampr Tetr28
    pFVP25ColEI, mob+, Ampr; source of the BamHI-PstI fragment harboring gfp56
    pBCSK+Cloning vector; CmrStratagene (La Jolla, Calif.)
    pMU125pWH1266 with gfp under control of the Tet promoterThis work
  • a Ampr, ampicillin resistant; Kmr, kanamycin resistant; Tetr, tetracycline resistant; RAPD, random amplified polymorphic DNA.