TABLE 4.

Major metabolites formed by cells of various L. brevis, L. paralimentarius, L. pontis, L. sanfranciscensis, and W. cibaria strains in culture supernatant after 16 h of fermentation at 30°Ca

StrainAmt of metabolite (mmol liter−1)Fructose fermentationbFQ (lactic acid/ acetic acid)
MaltoseFructoseLactic acidAcetic acidEthanolMannitol
L. brevis
    ACA-DC 3365−31 (1)−27.0 (0.3)58 (3)15 (1)32 (1)18.4 (0.8)Positive3.9 (0.2)
    ACA-DC 3368−30.5 (0.7)−27 (2)61 (2)13 (3)34 (2)19.7 (0.8)Positive4.7 (0.7)
    ACA-DC 3401−30.5 (0.2)−27.2 (0.1)60.7 (0.7)13 (3)35 (1)19 (2)Positive4.6 (0.9)
    ACA-DC 3404−30.3 (0.2)−29.5 (0.1)60.6 (0.4)15 (3)31.9 (0.9)19 (1)Positive4.2 (0.9)
    ACA-DC 3406−30.4 (0.2)−28.7 (0.6)62 (1)15 (3)35 (2)19 (1)Positive4.3 (0.9)
    ACA-DC 3410−30.3 (0.2)−26.9 (0.2)59.1 (0.8)16 (2)34 (2)18 (2)Positive3.6 (0.4)
    ACA-DC 3416−28 (3)−26 (2)57 (5)15 (5)29 (4)18 (1)Positive4 (2)
    ACA-DC 3420−30.8 (0.6)−29.7 (0.3)62.0 (0.7)15 (3)37 (4)20 (2)Positive4.3 (0.9)
    ACA-DC 4003−29.5 (0.1)−29 (2)61.7 (0.6)12.7 (0.5)38 (1)17.6 (0)Positive4.9 (0.2)
L. brevis-like
    ACA-DC 3411 t1−20 (1)−3 (3)49 (4)19 (9)21 (3)1.0 (0.1)Positive3 (1)
L. paralimentarius
    ACA-DC 3363−2.0 (0.7)−24 (6)48 (3)00.5 (0.3)3 (5)PositiveNRc
    ACA-DC 3413−15.8 (0.4)−15 (3)103 (2)00.7 (0.2)0.2 (0.4)PositiveNR
    ACA-DC 3415−1.40 (0.09)−22 (7)61.2 (0.3)00.10 (0.06)0.2 (0.4)PositiveNR
L. pontis
    LMG 14187T−18 (12)−26 (3)28 (22)16 (3)11 (9)24 (2)Positive2 (2)
    LMG 14188−19 (6)−26 (1)37 (12)16 (3)19 (12)23 (1)Positive2.3 (0.6)
L. sanfranciscensis
    ACA-DC 3370−31 (1)−29 (1)62.6 (0.7)28 (1)24 (2)25.1 (0.9)Negative2.25 (0.06)
    ACA-DC 3373−28 (2)−29.3 (0.8)65 (2)30 (3)20 (2)24 (1)Negative2.2 (0.2)
    ACA-DC 3378−27 (2)−28 (2)63 (1)26 (2)19 (2)25.5 (0.4)Negative2.4 (0.2)
    ACA-DC 3380−30.6 (0.9)−29.6 (0.4)63 (1)28.5 (0.9)23 (1)25 (2)Negative2.21 (0.03)
    ACA-DC 3382−30.2 (0.2)−29 (1)61.6 (0.3)29 (3)23 (2)26 (1)Negative2.2 (0.2)
    ACA-DC 3383−29 (1)−29.5 (0.1)58 (1)30 (5)20 (5)26 (2)Negative2.0 (0.4)
    ACA-DC 3408−31 (2)−29.9 (0.5)72 (4)28 (2)23 (3)23.2 (0.3)Negative2.6 (0.2)
    ACA-DC 3426−29.8 (0.2)−33.8 (0.1)64.7 (0.9)27 (2)20.3 (0.5)23.4 (0.7)Negative2.4 (0.2)
    LMG 11477−21 (2)−29.7 (0.3)45 (2)31 (2)12 (1)24 (3)Negative1.4 (0.2)
    LMG 16002T−17 (4)−29 (2)34 (4)17 (4)10 (2)24 (4)Negative2.0 (0.3)
    C57−28 (3)−1 (2)69 (7)19 (9)30 (4)0.1 (0.1)Negative4 (2)
    E13−28 (3)−2 (1)65 (4)13 (9)28 (2)0.1 (0.1)Negative6 (3)
W. cibaria
    ACA-DC 3385−27 (2)−11 (1)69.5 (0.6)3 (2)29 (3)−2 (1)Positive27 (12)
    ACA-DC 3387−30 (2)−13 (1)77 (2)3 (1)31 (2)0.0 (0.8)Positive25 (7)
    ACA-DC 3392−21.8 (0.7)−9.6 (0.7)56 (2)3 (2)26 (1)−2 (2)Positive19 (9)
    ACA-DC 3394−28 (1)−13 (2)71 (2)4.2 (0.7)30.9 (0.8)−1 (2)Positive17 (2)
    ACA-DC 3396−22 (1)−8.7 (0.2)58 (3)5 (2)26 (4)−1 (2)Positive14 (5)
    ACA-DC 3402−22 (2)−14 (2)65 (6)5 (5)29 (3)0.2 (0.6)Positive18 (10)
  • a All fermentations were carried out in mMRS medium (see note a to Table 1). All determinations were performed in triplicate. The standard deviation is given in parentheses. Negative values indicate consumption of substrates; positive values indicate products formed.

  • b Fructose fermentation was determined by analyzing the fructose consumption in the fermentation medium lacking maltose.

  • c NR, not relevant.