TABLE 2.

Characterization of transposome insertion derivatives of A. baumannii 19606

InsertionTarget siteGene, function disrupteda
1GCCCTAAAAdapA, dihydrodipicolinate synthase
2GATCATAGTgacS/lemA, histidine kinase sensor
4GTAGTGACTcsuB, chaperone-usher secretion system
5CTACAATCAgacS/lemA, histidine kinase sensor
9CGCGGATACtrpD, anthranilate phosphoribosyltransferase
10GTTTATTCAargF, ornithine carbamoyltransferase
11GAACATGATgacS/lemA, histidine kinase sensor
12GGGCCATACargG, argininosuccinate synthase
13ATAGAATGGaceElaceA, pyruvate dehydrogenase E1
15AATGGAAACproA, γ-glutamyl phosphate reductase
16AATATACGTcysI/nirA/sir, sulfite reductase
19CTACGATGCtrpE, anthranilate synthase
23CTATTCACAtrpE, anthranilate synthase
25GAACATGATgacS/lemA, histidine kinase sensor
26CATGTGAATcysI/nirA/sir, sulfite reductase
28CATGTGAATcysI/nirA/sir, sulfite reductase
30GCATTGGGThisA/his4, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
31CTGCAAACChisH, glutamine amidotransferase
44GTTTTACGTcysI/nirA/sir, sulfite reductase
144GTCACAAACcsuE, chaperone-usher secretion system
249ATGGTAAAAHypothetical sensor histidine kinase
  • a Potential genes and functions were predicted by BLASTp and BLASTx searches.