TABLE 2.

Plasmids and oligonucleotides used in this study

Plasmid or oligonucleotideRelevant characteristicsaSource or reference
Plasmids
    pGEM-TApr, PCR product cloning vector, 3 kbPromega
    pZErO-2Kanr, cloning vector, 3.3 kbInvitrogen
    pJMBYC01pGEM-T::100-bp skgA1 PCR product, 3.1 kbThis work
    pJMBYC02pZErO-2::2.1-kb HindIII fragment of a plasmid from L. sake 2512, 5.4 kbThis work
Oligonucleotides
    SakG015′ AAR TAT TAT GGN AAY GGN GT 3′
    SakG025′ ACA TGA TGN CCN CCR TTN GC 3′
  • a Apr, ampicillin resistant; Kanr, kanamycin resistant.