TABLE 2.

Oligonucleotide primers used in this study

PrimerSequence (5′-3′)aAdded restriction siteDesignationSource or accession no.
1GCGGATCCGCATGAGTGATCTAGACAGACTTBamHIlinA-senseD90355
2GCCTCGAGTTATGCGCCGGACGGTGCGAAATGXhoIlinA1-antisenseThis study
3GCCTCGAGTCACGATTTTTGCAACAGAGCXhoIlinA2-antisenseThis study
4GCGGATCCGCATGAGCCTCGGCGCAAAGCCABamHIlinB-senseD14594
5GCCTCGAGTTATGCTGGGCGCAATCGCCGGACXhoIlinB-antisenseD14594
6GCGGATCCGCATGTCTGATTTGAGCGGCEcoRIlinC-senseD14595
7GCCTCGAGTCAGATCGCGGTAAAGCCGCCGTCXhoIlinC-antisenseD14595
8GCGAATTCAATGAGCGCTGATACAGAAEcoRIlinD-senseD89733
9GCCTCGAGTTAGGCGTTGCTCAGGAGATGGATXhoIlinD-antisenseD89733
10GCGGATCCGCATGGCTAACAGACTCGCAGGCABamHIlinX-senseD23722
11GCCTCGAGTCAAACACCCACGGACCAGCCTCCXhoIlinX-antisenseD23722
12AGGAATTCCATGATGCAACTGCCCGAAEcoRIlinE-senseAB021867
13AGCTCGAGCTCAAATGACGATCGGATCXhoIlinE-antisenseAB021867
14TGGGATCCCCGTGAATATAGATGACCTGGBamHIlinR-senseAB021860
15GGGTCGACTCACATCCGCGCGGACAGSalIlinR-antisenseAB021860
16GCGGATCCGCTCTGTTGCAAAAATCGTGAAGCBamHITn610-senseX536535
17GCGGATCCGATGACCATGATTACGCCBamHITn610-antisenseX536535
  • a Restriction sites are underlined.